Nicotine exposure exacerbates development of cataracts in a type 1 diabetic rat model

Nima Tirgan, Gabriela A. Kulp, Praveena Gupta, Adam Boretsky, Tomasz A. Wiraszka, Bernard Godley, Ronald Tilton, Massoud Motamedi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Diabetes and smoking are known risk factors for cataract development. In this study, we evaluated the effect of nicotine on the progression of cataracts in a type 1 diabetic rat model. Diabetes was induced in Sprague-Dawley rats by a single injection of 65 mg/kg streptozotocin. Daily nicotine injections were administered subcutaneously. Forty-five rats were divided into groups of diabetics with and without nicotine treatment and controls with and without nicotine treatment. Progression of lens opacity was monitored using a slit lamp biomicroscope and scores were assigned. To assess whether systemic inflammation played a role in mediating cataractogenesis, we studied serum levels of eotaxin, IL-6, and IL-4. The levels of the measured cytokines increased significantly in nicotine-treated and untreated diabetic animals versus controls and demonstrated a positive trend in the nicotine-treated diabetic rats. Our data suggest the presence of a synergistic relationship between nicotine and diabetes that accelerated cataract formation via inflammatory mediators.

Original languageEnglish (US)
Article number349320
JournalExperimental Diabetes Research
Volume2012
DOIs
StatePublished - 2012

Fingerprint

Nicotine
Cataract
Injections
Streptozocin
Rat
Interleukin-4
Sprague Dawley Rats
Interleukin-6
Diabetes
Smoking
Cytokines
Inflammation
Therapeutics
Progression
Serum

ASJC Scopus subject areas

  • Medicine(all)
  • Endocrinology, Diabetes and Metabolism
  • Music

Cite this

Nicotine exposure exacerbates development of cataracts in a type 1 diabetic rat model. / Tirgan, Nima; Kulp, Gabriela A.; Gupta, Praveena; Boretsky, Adam; Wiraszka, Tomasz A.; Godley, Bernard; Tilton, Ronald; Motamedi, Massoud.

In: Experimental Diabetes Research, Vol. 2012, 349320, 2012.

Research output: Contribution to journalArticle

Tirgan, Nima ; Kulp, Gabriela A. ; Gupta, Praveena ; Boretsky, Adam ; Wiraszka, Tomasz A. ; Godley, Bernard ; Tilton, Ronald ; Motamedi, Massoud. / Nicotine exposure exacerbates development of cataracts in a type 1 diabetic rat model. In: Experimental Diabetes Research. 2012 ; Vol. 2012.
@article{685cfb8a9c1643329c4011163cbdd38f,
title = "Nicotine exposure exacerbates development of cataracts in a type 1 diabetic rat model",
abstract = "Diabetes and smoking are known risk factors for cataract development. In this study, we evaluated the effect of nicotine on the progression of cataracts in a type 1 diabetic rat model. Diabetes was induced in Sprague-Dawley rats by a single injection of 65 mg/kg streptozotocin. Daily nicotine injections were administered subcutaneously. Forty-five rats were divided into groups of diabetics with and without nicotine treatment and controls with and without nicotine treatment. Progression of lens opacity was monitored using a slit lamp biomicroscope and scores were assigned. To assess whether systemic inflammation played a role in mediating cataractogenesis, we studied serum levels of eotaxin, IL-6, and IL-4. The levels of the measured cytokines increased significantly in nicotine-treated and untreated diabetic animals versus controls and demonstrated a positive trend in the nicotine-treated diabetic rats. Our data suggest the presence of a synergistic relationship between nicotine and diabetes that accelerated cataract formation via inflammatory mediators.",
author = "Nima Tirgan and Kulp, {Gabriela A.} and Praveena Gupta and Adam Boretsky and Wiraszka, {Tomasz A.} and Bernard Godley and Ronald Tilton and Massoud Motamedi",
year = "2012",
doi = "10.1155/2012/349320",
language = "English (US)",
volume = "2012",
journal = "Experimental Diabesity Research",
issn = "1543-8600",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Nicotine exposure exacerbates development of cataracts in a type 1 diabetic rat model

AU - Tirgan, Nima

AU - Kulp, Gabriela A.

AU - Gupta, Praveena

AU - Boretsky, Adam

AU - Wiraszka, Tomasz A.

AU - Godley, Bernard

AU - Tilton, Ronald

AU - Motamedi, Massoud

PY - 2012

Y1 - 2012

N2 - Diabetes and smoking are known risk factors for cataract development. In this study, we evaluated the effect of nicotine on the progression of cataracts in a type 1 diabetic rat model. Diabetes was induced in Sprague-Dawley rats by a single injection of 65 mg/kg streptozotocin. Daily nicotine injections were administered subcutaneously. Forty-five rats were divided into groups of diabetics with and without nicotine treatment and controls with and without nicotine treatment. Progression of lens opacity was monitored using a slit lamp biomicroscope and scores were assigned. To assess whether systemic inflammation played a role in mediating cataractogenesis, we studied serum levels of eotaxin, IL-6, and IL-4. The levels of the measured cytokines increased significantly in nicotine-treated and untreated diabetic animals versus controls and demonstrated a positive trend in the nicotine-treated diabetic rats. Our data suggest the presence of a synergistic relationship between nicotine and diabetes that accelerated cataract formation via inflammatory mediators.

AB - Diabetes and smoking are known risk factors for cataract development. In this study, we evaluated the effect of nicotine on the progression of cataracts in a type 1 diabetic rat model. Diabetes was induced in Sprague-Dawley rats by a single injection of 65 mg/kg streptozotocin. Daily nicotine injections were administered subcutaneously. Forty-five rats were divided into groups of diabetics with and without nicotine treatment and controls with and without nicotine treatment. Progression of lens opacity was monitored using a slit lamp biomicroscope and scores were assigned. To assess whether systemic inflammation played a role in mediating cataractogenesis, we studied serum levels of eotaxin, IL-6, and IL-4. The levels of the measured cytokines increased significantly in nicotine-treated and untreated diabetic animals versus controls and demonstrated a positive trend in the nicotine-treated diabetic rats. Our data suggest the presence of a synergistic relationship between nicotine and diabetes that accelerated cataract formation via inflammatory mediators.

UR - http://www.scopus.com/inward/record.url?scp=84867385320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867385320&partnerID=8YFLogxK

U2 - 10.1155/2012/349320

DO - 10.1155/2012/349320

M3 - Article

VL - 2012

JO - Experimental Diabesity Research

JF - Experimental Diabesity Research

SN - 1543-8600

M1 - 349320

ER -