Ninety day toxicity study of chloroacetic acids in rats

Hari K. Bhat, Mary F. Kanz, Gerald Campbell, Ghulam Ansari

Research output: Contribution to journalArticle

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Abstract

Ninety Day Toxicity Study of Chloroacetic Acids in Rats. BHAT , H. K, KANZ, M. F., CAMPBELL G. A., AND ANSARI. G. A. S. (1991). Fundam. Appl Toxicol. 17, 240-253. Chloroacetic acids are produced in drinking water as a result of disinfection processes. Chloroacetic acids are also metabolites of widely usad and toxic halogenated hydrocarbons. Thus, chronic human exposure to these chemicals is likely to occur. The objective of the present study was to examine the toxic effects of monochloroacetic acid (MCA), dichloroacetic acid (DCA), and trichloroacetic acid (TCA) in a 90-day subchronic study in rats via oral exposure by drinking water. Chloroacetic acid solutions were prepared at concentrations which provided an approximate intake of 4-Jan the LD50 dose per day: MCA, 1.9 mM; DCA, 80.5 mM; TCA, 45.8 mM. Control rats received distilled water only. After 90 days, major organs were removed, fixed, paraffin embedded, and stained. Light microscopic examination of the major organs revealed variable degrees of alterations in the lung and liver of all three treated groups. In the liver, morphological changes were predominantly localized to the portal triads, which were mildly to moderately enlarged with random bile duct proliferation, extension of portal veins, fibrosis, edema, and occasional foci of inflammation. In the lungs, minimal alterations were observed as foci of perivascular inflammation on small pulmonary veins. Morphological changes in the testes and brain were seen only in the DCA treated group. Testes were atrophic with few spermatocytes and no mature spermatozoa. Focal vacuolation and gliosis were present in the forebrain and brainstem. The results of these studies indicate that, relative to their respective LD50 values DCA given at 80.5 mM is more toxic than TCA given at 45.8 mM and MCA at 1.9 mM is least toxic.

Original languageEnglish (US)
Pages (from-to)240-253
Number of pages14
JournalToxicological Sciences
Volume17
Issue number2
DOIs
StatePublished - Aug 1991
Externally publishedYes

Fingerprint

Chloroacetates
Dichloroacetic Acid
Poisons
Trichloroacetic Acid
Toxicity
Rats
Lethal Dose 50
Drinking Water
Liver
Testis
Halogenated Hydrocarbons
Rat control
Inflammation
Lung
Gliosis
Spermatocytes
Pulmonary Veins
Disinfection
Metabolites
Prosencephalon

ASJC Scopus subject areas

  • Molecular Biology
  • Embryology
  • Cell Biology
  • Genetics
  • Developmental Biology
  • Toxicology

Cite this

Ninety day toxicity study of chloroacetic acids in rats. / Bhat, Hari K.; Kanz, Mary F.; Campbell, Gerald; Ansari, Ghulam.

In: Toxicological Sciences, Vol. 17, No. 2, 08.1991, p. 240-253.

Research output: Contribution to journalArticle

Bhat, Hari K. ; Kanz, Mary F. ; Campbell, Gerald ; Ansari, Ghulam. / Ninety day toxicity study of chloroacetic acids in rats. In: Toxicological Sciences. 1991 ; Vol. 17, No. 2. pp. 240-253.
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