Nisoldepine inhibits formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) receptor-coupled calcium transport in human neutrophils

K. C. Williamson, A. I. Tauber, J. Navarro

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) dependent Ca2+ uptake by human neutrophils consists of at least two components, one of which is sensitive to dihydropyridine derivatives. Inhibition by dihydropyridine derivatives showed the rank order of nisoldepine > nitrendipine > nimodepine > /Bay K 8644. The nisoldepine-sensitive calcium uptake exhibited an ID50 of 1.5 μM and maximal inhibition were observed at 5 μM. Neither calcium efflux or [3H]fMet-Leu-Phe binding was affected by nisoldepine up to 10 μM. The inhibition by nisoldepine was inversely proportional to the extracellular calcium concentration. Unlabeled nisoldepine and other dihydropyridine derivatives displaced the specific binding of [3H]PN 200-110 to human neutrophils. Our data suggest a relationship between dihydropyridine binding and the inhibition of fMet-Leu-Phe-dependent Ca2+ uptake.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalJournal of Leukocyte Biology
Issue number3
StatePublished - 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


Dive into the research topics of 'Nisoldepine inhibits formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) receptor-coupled calcium transport in human neutrophils'. Together they form a unique fingerprint.

Cite this