Nitric oxide induces calcium-dependent [3H]dopamine release from striatal slices

G. Lonart, K. L. Cassels, K. M. Johnson

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Hydroxylamine (0.01-30 mM), a nitric oxide (NO) generator, produced a concentration-dependent release of [3H]dopamine ([3H]DA) from rat striatal slices. Hemoglobin (10 μM), a NO scavenger, reduced basal [3H]DA release and blocked hydroxylamine (100 μM)-stimulated [3H]DA efflux. Tetrodotoxin (0.5 μM) had no significant effect. Sodium cyanide was used as a model compound to test the possibility that NO acted through blockade of mitochondrial electron transport. Calcium-free experimental buffer (1 mM EGTA) reduced basal release and the hydroxylamine response, while sodium cyanide-induced release did not change under these experimental conditions. Cadmium (200 μM), a non-selective inhibitor of voltage-dependent calcium channels, reduced the hydroxylamine response by 69%. Methylene blue (10 μM), an inhibitor of guanylate cyclase, produced a 3-fold increase in the basal release but had no significant effect on the hydroxylamine response. These data suggest that NO induces calcium-dependent [3H]DA release from the striatum via a mechanism which is independent of blockade of electron transport or activation of guanylate cyclase.

Original languageEnglish (US)
Pages (from-to)192-198
Number of pages7
JournalJournal of Neuroscience Research
Volume35
Issue number2
DOIs
StatePublished - 1993

Fingerprint

Corpus Striatum
Hydroxylamine
Dopamine
Nitric Oxide
Calcium
Sodium Cyanide
Guanylate Cyclase
Electron Transport
Hemoglobin M
Methylene Blue
Egtazic Acid
Tetrodotoxin
Calcium Channels
Cadmium
Buffers

Keywords

  • cadmium
  • cyclic GMP
  • hemoglobin
  • hydroxylamine
  • methylene blue
  • sodium cyanide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nitric oxide induces calcium-dependent [3H]dopamine release from striatal slices. / Lonart, G.; Cassels, K. L.; Johnson, K. M.

In: Journal of Neuroscience Research, Vol. 35, No. 2, 1993, p. 192-198.

Research output: Contribution to journalArticle

Lonart, G. ; Cassels, K. L. ; Johnson, K. M. / Nitric oxide induces calcium-dependent [3H]dopamine release from striatal slices. In: Journal of Neuroscience Research. 1993 ; Vol. 35, No. 2. pp. 192-198.
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