Nitric oxide induces calcium-dependent [3H]dopamine release from striatal slices

G. Lonart, K. L. Cassels, K. M. Johnson

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Abstract

Hydroxylamine (0.01-30 mM), a nitric oxide (NO) generator, produced a concentration-dependent release of [3H]dopamine ([3H]DA) from rat striatal slices. Hemoglobin (10 μM), a NO scavenger, reduced basal [3H]DA release and blocked hydroxylamine (100 μM)-stimulated [3H]DA efflux. Tetrodotoxin (0.5 μM) had no significant effect. Sodium cyanide was used as a model compound to test the possibility that NO acted through blockade of mitochondrial electron transport. Calcium-free experimental buffer (1 mM EGTA) reduced basal release and the hydroxylamine response, while sodium cyanide-induced release did not change under these experimental conditions. Cadmium (200 μM), a non-selective inhibitor of voltage-dependent calcium channels, reduced the hydroxylamine response by 69%. Methylene blue (10 μM), an inhibitor of guanylate cyclase, produced a 3-fold increase in the basal release but had no significant effect on the hydroxylamine response. These data suggest that NO induces calcium-dependent [3H]DA release from the striatum via a mechanism which is independent of blockade of electron transport or activation of guanylate cyclase.

Original languageEnglish (US)
Pages (from-to)192-198
Number of pages7
JournalJournal of Neuroscience Research
Volume35
Issue number2
DOIs
StatePublished - 1993

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Keywords

  • cadmium
  • cyclic GMP
  • hemoglobin
  • hydroxylamine
  • methylene blue
  • sodium cyanide

ASJC Scopus subject areas

  • Neuroscience(all)

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