Nitric oxide induces neurotransmitter release from hippocampal slices

Gyorgy Lonart, Jia Wang, Kenneth M. Johnson

Research output: Contribution to journalArticle

264 Scopus citations

Abstract

Hydroxylamine (1-300 μM), a nitric oxide generator, stimulated the release of [3H]norepinephrine ([3H]NE) and [14C]acetylchoIine ([14C]ACh) from rat hippocampal slices in a concentration-dependent manner (EC50 = 30 μM). A maximally effective concentration of hydroxylamine (300 μM) produced a 24-fold increase in the basal [3H]NE and 3.6-fold increase in the in the basal [14C]ACh efflux. Sodium nitroprusside (SNP), also stimulated the release of [3H]NE, but only at high concentrations (10-30 mM). Calcium-free experimental buffer (1 mM EGTA) abolished the response. Hemoglobin (0.3 μM) inhibited the effect of 100 μM hydroxylamine in a manner which was specific for nitric oxide. In addition, 100 μM hydroxyiarnine increased the efflux of endogenous GABA and glutamate by 3- and 6-fold, respectively.

Original languageEnglish (US)
Pages (from-to)271-272
Number of pages2
JournalEuropean Journal of Pharmacology
Volume220
Issue number2-3
DOIs
StatePublished - Sep 22 1992

Keywords

  • Hydroxylamine
  • Neurotransmitter release
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Pharmacology

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