Nitric oxide localized to spinal cords of mice with experimental allergic encephalomyelitis

An electron paramagnetic resonance study

Robin F. Lin, Tien Sung Lin, Ronald Tilton, Anne H. Cross

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Experimental allergic encephalomyelitis (EAE) is a demyelinating autoimmune disorder that can be induced in susceptible mice by T lymphocytes sensitized to central nervous system (CNS) myelin components and is a prime animal model for the human CNS demyelinating disorder, multiple sclerosis (MS). Although CNS inflammation in which T lymphocytes and activated macrophages are the predominant cell types is observed in mice with EAE and in humans with MS, the exact mechanisms underlying the CNS damage and demyelination are not understood. Nitric oxide (NO), a gaseous free radical, has recently been shown to be a cytolytic product of activated macrophages. Using electron paramagnetic resonance spectroscopy, the nitric oxide free radical complexed with iron-sulfur proteins has been identified in affected spinal cords of mice with EAE, concurrent with the diminution of iron-sulfur proteins. These results indicate NO may play a role in the disease process of EAE, and perhaps MS.

Original languageEnglish (US)
Pages (from-to)643-648
Number of pages6
JournalJournal of Experimental Medicine
Volume178
Issue number2
StatePublished - Aug 1 1993
Externally publishedYes

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Autoimmune Experimental Encephalomyelitis
Electron Spin Resonance Spectroscopy
Spinal Cord
Nitric Oxide
Demyelinating Diseases
Iron-Sulfur Proteins
Multiple Sclerosis
Central Nervous System
Free Radicals
Macrophages
T-Lymphocytes
Central Nervous System Diseases
Myelin Sheath
Spectrum Analysis
Animal Models
Inflammation

ASJC Scopus subject areas

  • Immunology

Cite this

Nitric oxide localized to spinal cords of mice with experimental allergic encephalomyelitis : An electron paramagnetic resonance study. / Lin, Robin F.; Lin, Tien Sung; Tilton, Ronald; Cross, Anne H.

In: Journal of Experimental Medicine, Vol. 178, No. 2, 01.08.1993, p. 643-648.

Research output: Contribution to journalArticle

Lin, Robin F. ; Lin, Tien Sung ; Tilton, Ronald ; Cross, Anne H. / Nitric oxide localized to spinal cords of mice with experimental allergic encephalomyelitis : An electron paramagnetic resonance study. In: Journal of Experimental Medicine. 1993 ; Vol. 178, No. 2. pp. 643-648.
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