Nitric oxide (NO) increases expression of vascular endothelium growth factor (VEGF) in cultured cells

I. McMurtry, X. Zeng, S. Gebb, Csaba Szabo, I. Greenberg, J. Wispè

Research output: Contribution to journalArticle

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Abstract

VEGF is an endothelial cell-specific mitogen which stimulates angiogenesis in embryonic and adult tissues. Its expression in numerous cell types is upregulated by various signals including hypoxia and hypoglycemia. Because NO is an important vasoactive factor with gene regulatory activity, we have examined its effect on VEGF expression. Northern analysis showed that the NO donors, S-nitroso-N-acetyl-penicillamine (SNAP) and spermine-NO (0.01-1.0 mM), caused concentration- and time-dependent increases of VEGF mRNA in mouse fetal lung mesenchymal cells (MFLM). SNAP also increased VEGF in adult mouse lung epithelial cells and in both growing and quiescent bovine pulmonary artery smooth muscle cells. VEGF upregulation in MFLM was not mediated by cGMP, because the response to SNAP was neither potentiated by zaprinast nor mimicked by 8-br-cGMP. Actinomycin D (5 μg/ml)-chase experiments indicated that the upregulation was due at least partly to increased VEGF mRNA stability. These results raise the possibility that NO plays a role in angiogenesis hy stimul.iting VEGF expression.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

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Vascular Endothelium
endothelium
blood vessels
cultured cells
growth factors
nitric oxide
Cultured Cells
Intercellular Signaling Peptides and Proteins
Nitric Oxide
Cells
penicillamine
Penicillamine
lungs
angiogenesis
Lung
mice
Up-Regulation
Messenger RNA
actinomycin D
Nitric Oxide Donors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nitric oxide (NO) increases expression of vascular endothelium growth factor (VEGF) in cultured cells. / McMurtry, I.; Zeng, X.; Gebb, S.; Szabo, Csaba; Greenberg, I.; Wispè, J.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

McMurtry, I, Zeng, X, Gebb, S, Szabo, C, Greenberg, I & Wispè, J 1996, 'Nitric oxide (NO) increases expression of vascular endothelium growth factor (VEGF) in cultured cells', FASEB Journal, vol. 10, no. 3.
McMurtry, I. ; Zeng, X. ; Gebb, S. ; Szabo, Csaba ; Greenberg, I. ; Wispè, J. / Nitric oxide (NO) increases expression of vascular endothelium growth factor (VEGF) in cultured cells. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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AU - Wispè, J.

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