Nitric oxide production by cultured human aortic smooth muscle cells: Stimulation by fluid flow

M. Papadaki, Ronald Tilton, S. G. Eskin, L. V. McIntire

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

This study demonstrated that exposure of cultured human aortic smooth muscle cells (SMC) to fluid flow resulted in nitric oxide (NO) production, monitored by nitrite and guanosine 3',5'-cyclic monophosphate production. A rapid burst in nitrite production rate was followed by a more gradual increase throughout the period of flow exposure. Neither the initial burst nor the prolonged nitrite production was dependent on the level of shear stress in the range of 1.1-25 dyn/cm2. Repeated exposure to shear stress after a 30-min static period restimulated nitrite production similar to the initial burst. Ca2+-calmodulin antagonists blocked the initial burst in nitrite release. An inhibitor of nitric oxide synthase (NOS) blocked nitrite production, indicating that changes in nitrite reflect NO production. Treatment with dexamethasone or cycloheximide had no effect on nitrite production. Monoclonal antibodies directed against the inducible and endothelial NOS isoforms showed no immunoreactivity on Western blots, whereas monoclonal antibodies directed against the neuronal NOS gave specific products. These findings suggest that human aortic SMC express a constitutive neuronal NOS isoform, the enzymatic activity of which is modulated by flow.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume274
Issue number2
StatePublished - 1998
Externally publishedYes

Fingerprint

Nitrites
Smooth Muscle Myocytes
Nitric Oxide
Nitric Oxide Synthase Type I
Protein Isoforms
Monoclonal Antibodies
Nitric Oxide Synthase Type III
Cyclic GMP
Nitric Oxide Synthase Type II
Calmodulin
Cycloheximide
Nitric Oxide Synthase
Dexamethasone
Western Blotting

Keywords

  • Neuronal nitric oxide synthase
  • Shear stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Nitric oxide production by cultured human aortic smooth muscle cells : Stimulation by fluid flow. / Papadaki, M.; Tilton, Ronald; Eskin, S. G.; McIntire, L. V.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 274, No. 2, 1998.

Research output: Contribution to journalArticle

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