Nitric oxide regulates the polyol pathway of glucose metabolism in vascular smooth muscle cells

Kota Ramana, Deepak Chandra, Sanjay Srivastava, Aruni Bhatnagar, Satish Srivastava

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Increased reduction of glucose via the polyol pathway enzyme aldose reductase (AR) has been linked to the development of secondary diabetic complications. Because AR is a redox-sensitive protein, which in vitro is readily modified by NO donors, we tested the hypothesis that NO may be a physiological regulator of AR. We found that administration of the NO synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) increased sorbitol accumulation in the aorta of nondiabetic and diabetic rats, whereas treatment with L-arginine (a precursor of NO) or nitroglycerine patches prevented sorbitol accumulation. When incubated ex vivo with high glucose, sorbitol accumulation was increased by L-NAME and prevented by L-arginine in strips of aorta from rats or wild-type, but not eNOS-deficient, mice. Exposure to NO donors also inhibited AR and prevented sorbitol accumulation in rat aortic vascular smooth muscle cells (VSMC) in culture. The NO donors also increased the incorporation of radioactivity in the AR protein immunoprecipitated from VSMC in which the glutathione pool was prelabeled with [35S]-cysteine. Based on these observations, we suggest that NO regulates the vascular synthesis of polyols by S-thiolating AR; therefore, increasing NO synthesis or bioavailability may be useful in preventing diabetes-induced changes in the polyol pathway.

Original languageEnglish (US)
Pages (from-to)417-425
Number of pages9
JournalFASEB Journal
Volume17
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

aldehyde reductase
Aldehyde Reductase
polyols
Vascular Smooth Muscle
blood vessels
Metabolism
smooth muscle
myocytes
Smooth Muscle Myocytes
nitric oxide
Muscle
Nitric Oxide
Sorbitol
Cells
sorbitol
Glucose
glucose
metabolism
NG-Nitroarginine Methyl Ester
arginine

Keywords

  • Aldose reductase
  • Diabetes
  • Glutathiolation
  • Nitric oxide and nitrosothiols

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nitric oxide regulates the polyol pathway of glucose metabolism in vascular smooth muscle cells. / Ramana, Kota; Chandra, Deepak; Srivastava, Sanjay; Bhatnagar, Aruni; Srivastava, Satish.

In: FASEB Journal, Vol. 17, No. 3, 01.03.2003, p. 417-425.

Research output: Contribution to journalArticle

Ramana, Kota ; Chandra, Deepak ; Srivastava, Sanjay ; Bhatnagar, Aruni ; Srivastava, Satish. / Nitric oxide regulates the polyol pathway of glucose metabolism in vascular smooth muscle cells. In: FASEB Journal. 2003 ; Vol. 17, No. 3. pp. 417-425.
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