Nitric oxide synthase distribution during implantation in the mouse

T. L. Purcell, R. Given, K. Chwalisz, R. E. Garfield

    Research output: Contribution to journalArticle

    78 Citations (Scopus)

    Abstract

    The peri-implantation period is a critical time during murine development. Although the importance of nitric oxide has been demonstrated during gestation, its role in implantation has not been fully defined. The aim of this study was to quantify (by Western blotting) two prominent nitric oxide synthase (NOS) isoforms, inducible (iNOS) and endothelial (eNOS) and localize all three forms [iNOS, eNOS, and neuronal (nNOS)] by immunohistochemistry in uterine tissue from days 4 through 8 of pregnancy. By day 6, iNOS values were significantly elevated in implantation sites compared with interimplantation regions and continued to rise through day 8. Analysis of eNOS was similar, but implantation site values peaked by days 6 and 7. Labelled iNOS cells were within the decidua, around myometrial vessels, and within the ectoplacental cone. At implantation, eNOS was conspicuous, displaying label adjacent to the embryo in vessels of the primary decidual zone. nNOS was localized mainly in the mesometrium and myometrium and did not appear to change throughout the peri-implantation period. The increased iNOS and eNOS values following implantation in the embryonic site may imply roles in tissue remodelling, immunosuppression and vasoregulation. Nitric oxide may play an important role in the mechanisms of implantation where these factors are keys to successful pregnancy.

    Original languageEnglish (US)
    Pages (from-to)467-475
    Number of pages9
    JournalMolecular Human Reproduction
    Volume5
    Issue number5
    DOIs
    StatePublished - 1999

    Fingerprint

    Nitric Oxide Synthase
    Pregnancy
    Nitric Oxide
    Decidua
    Myometrium
    Nitric Oxide Synthase Type II
    Immunosuppression
    Protein Isoforms
    Embryonic Structures
    Western Blotting
    Immunohistochemistry

    Keywords

    • Decidua
    • Embryo
    • Implantation
    • Mouse
    • Nitric oxide synthase

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Genetics
    • Developmental Biology
    • Embryology
    • Cell Biology

    Cite this

    Nitric oxide synthase distribution during implantation in the mouse. / Purcell, T. L.; Given, R.; Chwalisz, K.; Garfield, R. E.

    In: Molecular Human Reproduction, Vol. 5, No. 5, 1999, p. 467-475.

    Research output: Contribution to journalArticle

    Purcell, TL, Given, R, Chwalisz, K & Garfield, RE 1999, 'Nitric oxide synthase distribution during implantation in the mouse', Molecular Human Reproduction, vol. 5, no. 5, pp. 467-475. https://doi.org/10.1093/molehr/5.5.467
    Purcell, T. L. ; Given, R. ; Chwalisz, K. ; Garfield, R. E. / Nitric oxide synthase distribution during implantation in the mouse. In: Molecular Human Reproduction. 1999 ; Vol. 5, No. 5. pp. 467-475.
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