Nitric oxide synthetase activity in cerebral post-ischemic reperfusion and effects of L-N(g)-nitroarginine and 7-nitroindazole on the survival

V. Sorrenti, C. Di Giacomo, A. Campisi, J. R. Perez-Polo, Angelo Vanella

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Nitric Oxide (NO) mediates a series of physiological processes including regulation of vascular tone, macrophage-mediated cytotoxicity, platelet aggregation, learning and long-term potentiation, neuronal transmission. Although NO mediates several physiological functions, overproduction of NO can be detrimental and play multiple roles in the pathophysiology of focal cerebral ischemia. In the present study NOS activities were evaluated in cerebellum and cerebral cortex of ischemic and post-ischemic reperfused rats using an experimental model of partial cerebral ischemia; moreover, the effects of L-N(G)Nitroarginine (NA, nonselective NOS inhibitor) or 7- Nitroindazole (7-NI, selective neuronal NOS inhibitor) administration were assayed on percentage survival of ischemic rats. An increase of NOS activity in the cerebellum and in cerebral cortex of ischemic and post-ischemic reperfused rats was observed. NA administration failed to induce neuroprotective effects, by increasing percentage of mortality of treated ischemic rats with respect to control group. In contrast, the treatment with the selective neuronal NOS inhibitor, 7-NI, induced a significant neuroprotective effect.

Original languageEnglish (US)
Pages (from-to)861-866
Number of pages6
JournalNeurochemical Research
Volume24
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Nitroarginine
Nitric Oxide Synthase
Reperfusion
Rats
Nitric Oxide
Neuroprotective Agents
Brain Ischemia
Cerebral Cortex
Cerebellum
Physiological Phenomena
Long-Term Potentiation
Macrophages
Cytotoxicity
Platelets
Platelet Aggregation
Blood Vessels
Theoretical Models
Agglomeration
Learning
Control Groups

Keywords

  • 7-NI
  • Cerebral ischemia
  • L-N(G)-NA
  • NOS

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Nitric oxide synthetase activity in cerebral post-ischemic reperfusion and effects of L-N(g)-nitroarginine and 7-nitroindazole on the survival. / Sorrenti, V.; Di Giacomo, C.; Campisi, A.; Perez-Polo, J. R.; Vanella, Angelo.

In: Neurochemical Research, Vol. 24, No. 7, 1999, p. 861-866.

Research output: Contribution to journalArticle

Sorrenti, V. ; Di Giacomo, C. ; Campisi, A. ; Perez-Polo, J. R. ; Vanella, Angelo. / Nitric oxide synthetase activity in cerebral post-ischemic reperfusion and effects of L-N(g)-nitroarginine and 7-nitroindazole on the survival. In: Neurochemical Research. 1999 ; Vol. 24, No. 7. pp. 861-866.
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