Nitrogen dioxide exposure: Effects on airway and blood cells

Mark W. Frampton, Joseph Boscia, Norbert J. Roberts, Mitra Azadniv, Alfonso Torres, C. O X Christopher, Paul E. Morrow, Joan Nichols, David Chalupa, Lauren M. Frasier, F. Raymond Gibb, Donna M. Speers, Ying Tsai, Mark J. Utell

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

This study examined the effects of nitrogen dioxide (NO2) exposure on airway inflammation, blood cells, and antiviral respiratory defense. Twenty-one healthy volunteers were exposed on separate occasions to air and 0.6 and 1.5 ppm NO2 for 3 h with intermittent moderate exercise. Phlebotomy and bronchoscopy were performed 3.5 h after each exposure, and recovered cells were challenged with respiratory viruses in vitro. Blood studies revealed a 4.1% NO2 dose-related decrease in hematocrit (P = 0.003). Circulating total lymphocytes (P = 0.024) and T lymphocytes (P = 0.049) decreased with NO2 exposure. Exposure to NO2 increased the blood lymphocyte CD4+-to-CD8+ ratio from 1.74 ± 0.11 to 1.85 ± 0.12 in males but decreased it from 1.88 ± 0.19 to 1.78 ± 0.19 in females (P < 0.001 for gender difference). Polymorphonuclear leukocytes in bronchial lavage increased with NO2 exposure (P = 0.003). Bronchial epithelial cells obtained after exposure to 1.5 ppm NO2 released 40% more lactate dehydrogenase after challenge with respiratory syncytial virus than with air exposure (P = 0.024). In healthy subjects, exposures to NO2 at levels found indoors cause mild airway inflammation, effects on blood cells, and increased susceptibility of airway epithelial cells to injury from respiratory viruses.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume282
Issue number1 26-1
StatePublished - 2002

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Nitrogen Dioxide
Blood Cells
Healthy Volunteers
Epithelial Cells
Air
Lymphocytes
Viruses
Inflammation
CD4-CD8 Ratio
Phlebotomy
Respiratory Syncytial Viruses
Bronchoscopy
Bronchoalveolar Lavage
Hematocrit
L-Lactate Dehydrogenase
Antiviral Agents
Neutrophils
Exercise
T-Lymphocytes
Wounds and Injuries

Keywords

  • Air pollution
  • Blood
  • Epithelial cells
  • Influenza virus
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Frampton, M. W., Boscia, J., Roberts, N. J., Azadniv, M., Torres, A., Christopher, C. O. X., ... Utell, M. J. (2002). Nitrogen dioxide exposure: Effects on airway and blood cells. American Journal of Physiology - Lung Cellular and Molecular Physiology, 282(1 26-1).

Nitrogen dioxide exposure : Effects on airway and blood cells. / Frampton, Mark W.; Boscia, Joseph; Roberts, Norbert J.; Azadniv, Mitra; Torres, Alfonso; Christopher, C. O X; Morrow, Paul E.; Nichols, Joan; Chalupa, David; Frasier, Lauren M.; Raymond Gibb, F.; Speers, Donna M.; Tsai, Ying; Utell, Mark J.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 282, No. 1 26-1, 2002.

Research output: Contribution to journalArticle

Frampton, MW, Boscia, J, Roberts, NJ, Azadniv, M, Torres, A, Christopher, COX, Morrow, PE, Nichols, J, Chalupa, D, Frasier, LM, Raymond Gibb, F, Speers, DM, Tsai, Y & Utell, MJ 2002, 'Nitrogen dioxide exposure: Effects on airway and blood cells', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 282, no. 1 26-1.
Frampton MW, Boscia J, Roberts NJ, Azadniv M, Torres A, Christopher COX et al. Nitrogen dioxide exposure: Effects on airway and blood cells. American Journal of Physiology - Lung Cellular and Molecular Physiology. 2002;282(1 26-1).
Frampton, Mark W. ; Boscia, Joseph ; Roberts, Norbert J. ; Azadniv, Mitra ; Torres, Alfonso ; Christopher, C. O X ; Morrow, Paul E. ; Nichols, Joan ; Chalupa, David ; Frasier, Lauren M. ; Raymond Gibb, F. ; Speers, Donna M. ; Tsai, Ying ; Utell, Mark J. / Nitrogen dioxide exposure : Effects on airway and blood cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2002 ; Vol. 282, No. 1 26-1.
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