Nitrosative stress induces a novel intra-S checkpoint pathway in Schizosaccharomyces pombe involving phosphorylation of Cdc2 by Wee1

Pranjal Biswas, Puranjoy Kar, Sanjay Ghosh

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Abstract Excess production of nitric oxide and reactive nitrogen intermediates causes nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study the cell cycle regulation under nitrosative stress response. We discovered a novel intra-S-phase checkpoint that is activated in S. pombe under nitrosative stress. The mechanism for this intra-S-phase checkpoint activation is distinctly different than previously reported for genotoxic stress in S. pombe by methyl methane sulfonate. Our flow cytometry data established the fact that Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. We checked the roles of Rad3, Rad17, Rad26, Swi1, Swi3, Cds1, and Chk1 under nitrosative stress but those were not involved in the activation of the DNA replication checkpoint. Rad24 was found to be involved in intra-S-phase checkpoint activation in S. pombe under nitrosative stress but that was independent of Cdc25.

Original languageEnglish (US)
Article number12436
Pages (from-to)145-155
Number of pages11
JournalFree Radical Biology and Medicine
Volume86
DOIs
StatePublished - Jul 9 2015
Externally publishedYes

Keywords

  • Cell cycle
  • Nitric oxide
  • Nitrosativestress
  • Replicationcheckpoint
  • Schizosaccharomycespombe

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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