@article{0763f3938995453ab02f317dc4d47c76,
title = "Nitrosative stress induces a novel intra-S checkpoint pathway in Schizosaccharomyces pombe involving phosphorylation of Cdc2 by Wee1",
abstract = "Abstract Excess production of nitric oxide and reactive nitrogen intermediates causes nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study the cell cycle regulation under nitrosative stress response. We discovered a novel intra-S-phase checkpoint that is activated in S. pombe under nitrosative stress. The mechanism for this intra-S-phase checkpoint activation is distinctly different than previously reported for genotoxic stress in S. pombe by methyl methane sulfonate. Our flow cytometry data established the fact that Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. We checked the roles of Rad3, Rad17, Rad26, Swi1, Swi3, Cds1, and Chk1 under nitrosative stress but those were not involved in the activation of the DNA replication checkpoint. Rad24 was found to be involved in intra-S-phase checkpoint activation in S. pombe under nitrosative stress but that was independent of Cdc25.",
keywords = "Cell cycle, Nitric oxide, Nitrosativestress, Replicationcheckpoint, Schizosaccharomycespombe",
author = "Pranjal Biswas and Puranjoy Kar and Sanjay Ghosh",
note = "Funding Information: We thank Prof. Kathy Gould (Vanderbilt University, USA), Prof. Antony Carr (University of Sussex, UK), Prof. Timothy Humphrey (University of Oxford, UK), and Prof. Paul Russell (The Scripps Research Institute, USA) for kindly providing the yeast strains used in this study. We thank Dr. Sanjaya Mallick and Dr. Debajit Bhowmik (at the University of Calcutta-BD Flow cytometry facility at CRNN, University of Calcutta) for their help in interpreting the flow cytometry data. The consumable for the entire work was supported by DBT, Govt. of India , Grant BT/PR12551/BRB/10/02/2009 dated September 3, 2010. We thank CRNN, University of Calcutta, DBT-IPLS, UPE, DAE, and UGC CAS Phase II Govt. of India for providing the infrastructural facility. CSIR, Govt. of India, Grant no. 09/028(0809)/2010-EMR-I dated 07.02.2011 and UGC, Govt. of India , Grant no. UGC/722/Jr. Fellow (Sc.) dated 23.04.2014 are acknowledged for providing fellowships to P.B. and P.K., respectively. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
month = jul,
day = "9",
doi = "10.1016/j.freeradbiomed.2015.05.021",
language = "English (US)",
volume = "86",
pages = "145--155",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
}