NMR-based investigations into target DNA search processes of proteins

Junji Iwahara, Levani Zandarashvili, Catherine A. Kemme, Alexandre Esadze

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations

Abstract

To perform their function, transcription factors and DNA-repair/modifying enzymes must first locate their targets in the vast presence of nonspecific, but structurally similar sites on genomic DNA. Before reaching their targets, these proteins stochastically scan DNA and dynamically move from one site to another on DNA. Solution NMR spectroscopy provides unique atomic-level insights into the dynamic DNA-scanning processes, which are difficult to gain by any other experimental means. In this review, we provide an introductory overview on the NMR methods for the structural, dynamic, and kinetic investigations of target DNA search by proteins. We also discuss advantages and disadvantages of these NMR methods over other methods such as single-molecule techniques and biochemical approaches.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalMethods
Volume148
DOIs
StatePublished - Sep 15 2018

Keywords

  • DNA scanning
  • Dynamics
  • Kinetics
  • NMR
  • Protein-DNA interactions
  • Target search

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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