Abstract
To perform their function, transcription factors and DNA-repair/modifying enzymes must first locate their targets in the vast presence of nonspecific, but structurally similar sites on genomic DNA. Before reaching their targets, these proteins stochastically scan DNA and dynamically move from one site to another on DNA. Solution NMR spectroscopy provides unique atomic-level insights into the dynamic DNA-scanning processes, which are difficult to gain by any other experimental means. In this review, we provide an introductory overview on the NMR methods for the structural, dynamic, and kinetic investigations of target DNA search by proteins. We also discuss advantages and disadvantages of these NMR methods over other methods such as single-molecule techniques and biochemical approaches.
| Original language | English (US) |
|---|---|
| Journal | Methods |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
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Keywords
- DNA scanning
- Dynamics
- Kinetics
- NMR
- Protein-DNA interactions
- Target search
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
NMR-based investigations into target DNA search processes of proteins. / Iwahara, Junji; Zandarashvili, Levani; Kemme, Catherine A.; Esadze, Alexandre.
In: Methods, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - NMR-based investigations into target DNA search processes of proteins
AU - Iwahara, Junji
AU - Zandarashvili, Levani
AU - Kemme, Catherine A.
AU - Esadze, Alexandre
PY - 2018/1/1
Y1 - 2018/1/1
N2 - To perform their function, transcription factors and DNA-repair/modifying enzymes must first locate their targets in the vast presence of nonspecific, but structurally similar sites on genomic DNA. Before reaching their targets, these proteins stochastically scan DNA and dynamically move from one site to another on DNA. Solution NMR spectroscopy provides unique atomic-level insights into the dynamic DNA-scanning processes, which are difficult to gain by any other experimental means. In this review, we provide an introductory overview on the NMR methods for the structural, dynamic, and kinetic investigations of target DNA search by proteins. We also discuss advantages and disadvantages of these NMR methods over other methods such as single-molecule techniques and biochemical approaches.
AB - To perform their function, transcription factors and DNA-repair/modifying enzymes must first locate their targets in the vast presence of nonspecific, but structurally similar sites on genomic DNA. Before reaching their targets, these proteins stochastically scan DNA and dynamically move from one site to another on DNA. Solution NMR spectroscopy provides unique atomic-level insights into the dynamic DNA-scanning processes, which are difficult to gain by any other experimental means. In this review, we provide an introductory overview on the NMR methods for the structural, dynamic, and kinetic investigations of target DNA search by proteins. We also discuss advantages and disadvantages of these NMR methods over other methods such as single-molecule techniques and biochemical approaches.
KW - DNA scanning
KW - Dynamics
KW - Kinetics
KW - NMR
KW - Protein-DNA interactions
KW - Target search
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UR - http://www.scopus.com/inward/citedby.url?scp=85047067601&partnerID=8YFLogxK
U2 - 10.1016/j.ymeth.2018.05.004
DO - 10.1016/j.ymeth.2018.05.004
M3 - Article
JO - Methods
JF - Methods
SN - 1046-2023
ER -