NMR-based investigations into target DNA search processes of proteins

Junji Iwahara, Levani Zandarashvili, Catherine A. Kemme, Alexandre Esadze

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To perform their function, transcription factors and DNA-repair/modifying enzymes must first locate their targets in the vast presence of nonspecific, but structurally similar sites on genomic DNA. Before reaching their targets, these proteins stochastically scan DNA and dynamically move from one site to another on DNA. Solution NMR spectroscopy provides unique atomic-level insights into the dynamic DNA-scanning processes, which are difficult to gain by any other experimental means. In this review, we provide an introductory overview on the NMR methods for the structural, dynamic, and kinetic investigations of target DNA search by proteins. We also discuss advantages and disadvantages of these NMR methods over other methods such as single-molecule techniques and biochemical approaches.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Nuclear magnetic resonance
DNA
Proteins
DNA Repair Enzymes
Structural dynamics
Nuclear magnetic resonance spectroscopy
Transcription Factors
Magnetic Resonance Spectroscopy
Scanning
Molecules
Kinetics

Keywords

  • DNA scanning
  • Dynamics
  • Kinetics
  • NMR
  • Protein-DNA interactions
  • Target search

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

NMR-based investigations into target DNA search processes of proteins. / Iwahara, Junji; Zandarashvili, Levani; Kemme, Catherine A.; Esadze, Alexandre.

In: Methods, 01.01.2018.

Research output: Contribution to journalArticle

Iwahara, Junji ; Zandarashvili, Levani ; Kemme, Catherine A. ; Esadze, Alexandre. / NMR-based investigations into target DNA search processes of proteins. In: Methods. 2018.
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