NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition

Munia Mukherjee, Kaushik Dutta, Mark White, David Cowburn, Robert O. Fox

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Flaviviruses cause many human diseases, including dengue fever, yellow fever, West Nile viral encephalitis, and hemorrhagic fevers, and are transmitted to their vertebrate hosts by infected mosquitoes and ticks. Domain III of the envelope protein (E-D3) is considered to be the primary viral determinant involved in the virus-host-cell receptor interaction, and thus represents an excellent target for antiviral drug development. Langat (LGT) virus is a naturally attenuated BSL-2 TBE virus and is a model for the pathogenic BSL-3 and BSL-4 viruses in the serogroup. We have determined the solution structure of LGTE-D3 using heteronuclear NMR spectroscopy. The backbone dynamics of LGT-E-D3 have been investigated using 15N relaxation measurements. A detailed analysis of the solution structure and dynamics of LGT-E-D3 suggests potential residues that could form a surface for molecular recognition, and thereby represent a target site for antiviral therapeutics design.

Original languageEnglish (US)
Pages (from-to)1342-1355
Number of pages14
JournalProtein Science
Volume15
Issue number6
DOIs
StatePublished - Jun 2006

Fingerprint

Arthropod Proteins
Flavivirus
Molecular recognition
Viruses
Nuclear magnetic resonance
Antiviral Agents
Viral Hemorrhagic Fevers
Viral Encephalitis
Tick-Borne Encephalitis Viruses
Biomolecular Nuclear Magnetic Resonance
Yellow Fever
Dengue
Ticks
Culicidae
Cell Communication
Vertebrates
Magnetic Resonance Spectroscopy
Binding energy
Nuclear magnetic resonance spectroscopy
Proteins

Keywords

  • Backbone dynamics
  • E protein
  • E protein domain III
  • Flavivirus
  • Langat virus
  • NMR relaxation
  • NMR spectroscopy
  • Protein conformation
  • Protein-protein interactions
  • Residual dipolar coupling

ASJC Scopus subject areas

  • Biochemistry

Cite this

NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition. / Mukherjee, Munia; Dutta, Kaushik; White, Mark; Cowburn, David; Fox, Robert O.

In: Protein Science, Vol. 15, No. 6, 06.2006, p. 1342-1355.

Research output: Contribution to journalArticle

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