NMR Studies on an Oligodeoxynucleotide Containing 2-Aminopurine opposite Adenine

G. Victor Fazakerley, Lawrence C. Sowers, Ramon Eritja, Bruce E. kaplan, Myron F. Goodman

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A heteroduplex containing the mismatch 2-aminopurine (AP)-adenine has been synthesized and studied by proton NMR. The mismatch was incorporated into the seuquence d[CGG(AP)GGC].d-(GCCACCG). One-dimensional nuclear Overhauser effect measurements in H20 and two-dimensional nuclear Overhauser effect spectra in D20 show AP-A base pairs in a wobble structure in which both bases are in the anti conformation. The adenine is stacked well in the helix, but the helix twist between the adenine and neighboring cytosine in the 3/direction is unusually small. As a result, the aminopurine on the opposite strand is somewhat pushed out of the helix. From the measurements of the imino proton line widths, the two adjacent G-C base pairs are not found to be significantly destabilized by the presence of the purine-purine wobble pair.

Original languageEnglish (US)
Pages (from-to)5641-5646
Number of pages6
Issue number18
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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