Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells

Smarajit Chakraborty, Ricksen S. Winardhi, Leslie K. Morgan, Jie Yan, Linda Kenney

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Unlike eukaryotes, bacteria undergo large changes in osmolality and cytoplasmic pH. It has been described that during acid stress, bacteria internal pH promptly acidifies, followed by recovery. Here, using pH imaging in single living cells, we show that following acid stress, bacteria maintain an acidic cytoplasm and the osmotic stress transcription factor OmpR is required for acidification. The activation of this response is non-canonical, involving a regulatory mechanism requiring the OmpR cognate kinase EnvZ, but not OmpR phosphorylation. Single cell analysis further identifies an intracellular pH threshold ~6.5. Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. During osmotic stress, the internal pH is above the threshold, triggering distinct OmpR-related pathways. Preventing intracellular acidification of Salmonella renders it avirulent, suggesting that acid stress pathways represent a potential therapeutic target. These results further emphasize the advantages of single cell analysis over studies of population averages.

Original languageEnglish (US)
Article number1587
JournalNature communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

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Osmoregulation
Chemical activation
activation
Bacteria
acids
Acids
Acidification
cells
Single-Cell Analysis
bacteria
Osmotic Pressure
Phosphorylation
Salmonella
thresholds
Dimerization
eukaryotes
salmonella
phosphorylation
Transcription Factors
Phosphotransferases

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells. / Chakraborty, Smarajit; Winardhi, Ricksen S.; Morgan, Leslie K.; Yan, Jie; Kenney, Linda.

In: Nature communications, Vol. 8, No. 1, 1587, 01.12.2017.

Research output: Contribution to journalArticle

Chakraborty, Smarajit ; Winardhi, Ricksen S. ; Morgan, Leslie K. ; Yan, Jie ; Kenney, Linda. / Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells. In: Nature communications. 2017 ; Vol. 8, No. 1.
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