Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy

Noël J C Bauer, Massoud Motamedi, James P. Wicksted, Wayne F. March, Carrol A B Webers, Fred Hendrikse

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A Laser Scanning Confocal Raman Spectroscopy (LSCRS) system was applied for the non-invasive quantification of the transport of a drug through the rabbit cornea in vivo. Employing LSCRS, the changes in the amplitude of a drug-specific Raman signal were assessed over time in the tearfilm and corneal epithelium of the living rabbit eye (n=6), after topical application of 25 μL·Trusopt 2%(TM). This allowed for quantification of pharmacokinetic variables. The effect of the drug on corneal hydration was also monitored. LSCRS demonstrated adequate sensitivity and reproducibility, for continuous real-time monitoring of the Trusopt concentration. Each concentration-time curve had a bi-phasic trend; the rapid initial phase (t<8 min.) corresponds to the nonproductive losses of Trusopt from the tears (k10=0.24±0.04 min- 1), and the slower later phase (t>20 min.) is the result of transfer of the drug from the corneal epithelium to the stroma (k23=0.0047±0.0004 min- 1). Drug absorption into the corneal epithelium occurred at a rate of k12=0.034±0.006 min-1. Trusopt caused an acute dehydrating effect, with a maximum decrease in corneal hydration of ~15% at ~60 min. following application of the drug. LSCRS has the specificity, sensitivity, reproducibility and spatial resolution for employment as a potentially valuable tool for the study of ocular pharmacokinetics.

Original languageEnglish (US)
Pages (from-to)123-134
Number of pages12
JournalJournal of Ocular Pharmacology and Therapeutics
Volume15
Issue number2
StatePublished - Apr 1999

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Raman Spectrum Analysis
Pharmacokinetics
dorzolamide
Corneal Epithelium
Lasers
Pharmaceutical Preparations
Rabbits
Cornea
Sensitivity and Specificity

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Bauer, N. J. C., Motamedi, M., Wicksted, J. P., March, W. F., Webers, C. A. B., & Hendrikse, F. (1999). Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy. Journal of Ocular Pharmacology and Therapeutics, 15(2), 123-134.

Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy. / Bauer, Noël J C; Motamedi, Massoud; Wicksted, James P.; March, Wayne F.; Webers, Carrol A B; Hendrikse, Fred.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 15, No. 2, 04.1999, p. 123-134.

Research output: Contribution to journalArticle

Bauer, NJC, Motamedi, M, Wicksted, JP, March, WF, Webers, CAB & Hendrikse, F 1999, 'Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy', Journal of Ocular Pharmacology and Therapeutics, vol. 15, no. 2, pp. 123-134.
Bauer, Noël J C ; Motamedi, Massoud ; Wicksted, James P. ; March, Wayne F. ; Webers, Carrol A B ; Hendrikse, Fred. / Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy. In: Journal of Ocular Pharmacology and Therapeutics. 1999 ; Vol. 15, No. 2. pp. 123-134.
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