Non-invasive assessment of ocular pharmacokinetics using confocal Raman spectroscopy

Noël J.C. Bauer, Massoud Motamedi, James P. Wicksted, Wayne F. March, Carrol A.B. Webers, Fred Hendrikse

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A Laser Scanning Confocal Raman Spectroscopy (LSCRS) system was applied for the non-invasive quantification of the transport of a drug through the rabbit cornea in vivo. Employing LSCRS, the changes in the amplitude of a drug-specific Raman signal were assessed over time in the tearfilm and corneal epithelium of the living rabbit eye (n=6), after topical application of 25 μL·Trusopt 2%(TM). This allowed for quantification of pharmacokinetic variables. The effect of the drug on corneal hydration was also monitored. LSCRS demonstrated adequate sensitivity and reproducibility, for continuous real-time monitoring of the Trusopt concentration. Each concentration-time curve had a bi-phasic trend; the rapid initial phase (t<8 min.) corresponds to the nonproductive losses of Trusopt from the tears (k10=0.24±0.04 min- 1), and the slower later phase (t>20 min.) is the result of transfer of the drug from the corneal epithelium to the stroma (k23=0.0047±0.0004 min- 1). Drug absorption into the corneal epithelium occurred at a rate of k12=0.034±0.006 min-1. Trusopt caused an acute dehydrating effect, with a maximum decrease in corneal hydration of ~15% at ~60 min. following application of the drug. LSCRS has the specificity, sensitivity, reproducibility and spatial resolution for employment as a potentially valuable tool for the study of ocular pharmacokinetics.

Original languageEnglish (US)
Pages (from-to)123-134
Number of pages12
JournalJournal of Ocular Pharmacology and Therapeutics
Volume15
Issue number2
DOIs
StatePublished - Apr 1999

ASJC Scopus subject areas

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)

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