Non-invasive assessment of the axial hydration gradient after corneal abrasion of the rabbit eye

N. J C Dauer, S. J. Koons, J. P. Wicksted, F. H M Jongsma, F. Hendrikse, Massoud Motamedi, W. F. March

Research output: Contribution to journalArticle

Abstract

Purpose. The lack of assessment and thus control of the hydration of the ablation site, is believed to he the cause of several undesired side-effects associated with cornea ablation. The aim of (his study was to quantify in-vivo the water distribution across the rabbit cornea as function of time after abrasion. Methods. A non-invasive scanning confocal Raman spectroscopy (SCRS) technique (25 mW Argon, Is probing time, SPKX500M, axial resolution 50 um) was used to assess the water content (Raman intensity ratio of water-peak/protein-peak) of consecutive layers along the optical axis of the cornea with scan-steps of 50 HID. Two regions were studied closely: the superficial 100 Jim (A) and the most posterior UK) u.m (P) of stroma. This technique was validated and calibrated in 9 m-vitro rabhil eyes, by quantifying the relationship between pachymetric- and SCRSassessed total corncal hydration (H). Eight corneas of NZW rabbits were abraded in-vivo, and the time dependenf changes in hydration were assessed for H, A and P. Results. Calibration revealed a linear relationship between pachyinelric- and SCRS-assessed H, invitrti (SI>=0.01, R-=0.94) as well as in-vivo before abrasion (SI)=0.10, R3=0.77). In agreement with current literature, H, A and P show different non-linear time-dependencies after abrasion. A correlated better with H than P <A=[0.72±0.()8]/J. R2=0.88. and /'=| 1.56±0.i 1 ]//, R:=<>.68), while the linear correlation between pachymetric- and SCRSassessed H after abrasion was reasonable (SD=().l 1, R2=0.87). Conclusion. Our results suggest that SCRS can be used to quantify the expected time-dependent changes of the axial corneal hydration gradient after abrasion m-vivo. This may render future applications of this technique possible in the assessment and control of corneal hydration.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997
Externally publishedYes

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Cornea
Raman Spectrum Analysis
Rabbits
Water
Argon
Calibration
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Dauer, N. J. C., Koons, S. J., Wicksted, J. P., Jongsma, F. H. M., Hendrikse, F., Motamedi, M., & March, W. F. (1997). Non-invasive assessment of the axial hydration gradient after corneal abrasion of the rabbit eye. Investigative Ophthalmology and Visual Science, 38(4).

Non-invasive assessment of the axial hydration gradient after corneal abrasion of the rabbit eye. / Dauer, N. J C; Koons, S. J.; Wicksted, J. P.; Jongsma, F. H M; Hendrikse, F.; Motamedi, Massoud; March, W. F.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

Dauer, N. J C ; Koons, S. J. ; Wicksted, J. P. ; Jongsma, F. H M ; Hendrikse, F. ; Motamedi, Massoud ; March, W. F. / Non-invasive assessment of the axial hydration gradient after corneal abrasion of the rabbit eye. In: Investigative Ophthalmology and Visual Science. 1997 ; Vol. 38, No. 4.
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abstract = "Purpose. The lack of assessment and thus control of the hydration of the ablation site, is believed to he the cause of several undesired side-effects associated with cornea ablation. The aim of (his study was to quantify in-vivo the water distribution across the rabbit cornea as function of time after abrasion. Methods. A non-invasive scanning confocal Raman spectroscopy (SCRS) technique (25 mW Argon, Is probing time, SPKX500M, axial resolution 50 um) was used to assess the water content (Raman intensity ratio of water-peak/protein-peak) of consecutive layers along the optical axis of the cornea with scan-steps of 50 HID. Two regions were studied closely: the superficial 100 Jim (A) and the most posterior UK) u.m (P) of stroma. This technique was validated and calibrated in 9 m-vitro rabhil eyes, by quantifying the relationship between pachymetric- and SCRSassessed total corncal hydration (H). Eight corneas of NZW rabbits were abraded in-vivo, and the time dependenf changes in hydration were assessed for H, A and P. Results. Calibration revealed a linear relationship between pachyinelric- and SCRS-assessed H, invitrti (SI>=0.01, R-=0.94) as well as in-vivo before abrasion (SI)=0.10, R3=0.77). In agreement with current literature, H, A and P show different non-linear time-dependencies after abrasion. A correlated better with H than P <A=[0.72±0.()8]/J. R2=0.88. and /'=| 1.56±0.i 1 ]//, R:=<>.68), while the linear correlation between pachymetric- and SCRSassessed H after abrasion was reasonable (SD=().l 1, R2=0.87). Conclusion. Our results suggest that SCRS can be used to quantify the expected time-dependent changes of the axial corneal hydration gradient after abrasion m-vivo. This may render future applications of this technique possible in the assessment and control of corneal hydration.",
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T1 - Non-invasive assessment of the axial hydration gradient after corneal abrasion of the rabbit eye

AU - Dauer, N. J C

AU - Koons, S. J.

AU - Wicksted, J. P.

AU - Jongsma, F. H M

AU - Hendrikse, F.

AU - Motamedi, Massoud

AU - March, W. F.

PY - 1997

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N2 - Purpose. The lack of assessment and thus control of the hydration of the ablation site, is believed to he the cause of several undesired side-effects associated with cornea ablation. The aim of (his study was to quantify in-vivo the water distribution across the rabbit cornea as function of time after abrasion. Methods. A non-invasive scanning confocal Raman spectroscopy (SCRS) technique (25 mW Argon, Is probing time, SPKX500M, axial resolution 50 um) was used to assess the water content (Raman intensity ratio of water-peak/protein-peak) of consecutive layers along the optical axis of the cornea with scan-steps of 50 HID. Two regions were studied closely: the superficial 100 Jim (A) and the most posterior UK) u.m (P) of stroma. This technique was validated and calibrated in 9 m-vitro rabhil eyes, by quantifying the relationship between pachymetric- and SCRSassessed total corncal hydration (H). Eight corneas of NZW rabbits were abraded in-vivo, and the time dependenf changes in hydration were assessed for H, A and P. Results. Calibration revealed a linear relationship between pachyinelric- and SCRS-assessed H, invitrti (SI>=0.01, R-=0.94) as well as in-vivo before abrasion (SI)=0.10, R3=0.77). In agreement with current literature, H, A and P show different non-linear time-dependencies after abrasion. A correlated better with H than P <A=[0.72±0.()8]/J. R2=0.88. and /'=| 1.56±0.i 1 ]//, R:=<>.68), while the linear correlation between pachymetric- and SCRSassessed H after abrasion was reasonable (SD=().l 1, R2=0.87). Conclusion. Our results suggest that SCRS can be used to quantify the expected time-dependent changes of the axial corneal hydration gradient after abrasion m-vivo. This may render future applications of this technique possible in the assessment and control of corneal hydration.

AB - Purpose. The lack of assessment and thus control of the hydration of the ablation site, is believed to he the cause of several undesired side-effects associated with cornea ablation. The aim of (his study was to quantify in-vivo the water distribution across the rabbit cornea as function of time after abrasion. Methods. A non-invasive scanning confocal Raman spectroscopy (SCRS) technique (25 mW Argon, Is probing time, SPKX500M, axial resolution 50 um) was used to assess the water content (Raman intensity ratio of water-peak/protein-peak) of consecutive layers along the optical axis of the cornea with scan-steps of 50 HID. Two regions were studied closely: the superficial 100 Jim (A) and the most posterior UK) u.m (P) of stroma. This technique was validated and calibrated in 9 m-vitro rabhil eyes, by quantifying the relationship between pachymetric- and SCRSassessed total corncal hydration (H). Eight corneas of NZW rabbits were abraded in-vivo, and the time dependenf changes in hydration were assessed for H, A and P. Results. Calibration revealed a linear relationship between pachyinelric- and SCRS-assessed H, invitrti (SI>=0.01, R-=0.94) as well as in-vivo before abrasion (SI)=0.10, R3=0.77). In agreement with current literature, H, A and P show different non-linear time-dependencies after abrasion. A correlated better with H than P <A=[0.72±0.()8]/J. R2=0.88. and /'=| 1.56±0.i 1 ]//, R:=<>.68), while the linear correlation between pachymetric- and SCRSassessed H after abrasion was reasonable (SD=().l 1, R2=0.87). Conclusion. Our results suggest that SCRS can be used to quantify the expected time-dependent changes of the axial corneal hydration gradient after abrasion m-vivo. This may render future applications of this technique possible in the assessment and control of corneal hydration.

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