Non-Invasive Transcranial Nano-Pulsed Laser Therapy Ameliorates Cognitive Function and Prevents Aberrant Migration of Neural Progenitor Cells in the Hippocampus of Rats Subjected to Traumatic Brain Injury

Emanuele Mocciaro, Auston Grant, Rinat O. Esenaliev, Irene Y. Petrov, Yuriy Petrov, Stacy L. Sell, Nicole L. Hausser, Jutatip Guptarak, Elizabeth Bishop, Margaret A. Parsley, Ian J. Bolding, Kathia M. Johnson, Maxwell Lidstone, Donald S. Prough, Maria Adelaide Micci

Research output: Contribution to journalArticle

Abstract

Traumatic brain injury (TBI) can lead to chronic diseases, including neurodegenerative disorders and epilepsy. The hippocampus, one of the most affected brain region after TBI, plays a critical role in learning and memory and is one of the only two regions in the brain in which new neurons are generated throughout life from neural stem cells (NSC) in the dentate gyrus (DG). These cells migrate into the granular layer where they integrate into the hippocampus circuitry. While increased proliferation of NSC in the hippocampus is known to occur shortly after injury, reduced neuronal maturation and aberrant migration of progenitor cells in the hilus contribute to cognitive and neurological dysfunctions, including epilepsy. Here, we tested the ability of a novel, proprietary non-invasive nano-pulsed laser therapy (NPLT), that combines near-infrared laser light (808 nm) and laser-generated, low-energy optoacoustic waves, to mitigate TBI-driven impairments in neurogenesis and cognitive function in the rat fluid percussion injury model. We show that injured rats treated with NPLT performed significantly better in a hippocampus-dependent cognitive test than did sham rats. In the DG, NPLT significantly decreased TBI-dependent impaired maturation and aberrant migration of neural progenitors, while preventing TBI-induced upregulation of specific microRNAs (miRNAs) in NSC. NPLT did not significantly reduce TBI-induced microglia activation in the hippocampus. Our data strongly suggest that NPLT has the potential to be an effective therapeutic tool for the treatment of TBI-induced cognitive dysfunction and dysregulation of neurogenesis, and point to modulation of miRNAs as a possible mechanism mediating its neuroprotective effects.

Original languageEnglish (US)
Pages (from-to)1108-1123
Number of pages16
JournalJournal of neurotrauma
Volume37
Issue number8
DOIs
StatePublished - Apr 15 2020

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Keywords

  • cognitive function
  • hippocampus
  • neuronal progenitors
  • optoacoustic laser therapy
  • TBI

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Mocciaro, E., Grant, A., Esenaliev, R. O., Petrov, I. Y., Petrov, Y., Sell, S. L., Hausser, N. L., Guptarak, J., Bishop, E., Parsley, M. A., Bolding, I. J., Johnson, K. M., Lidstone, M., Prough, D. S., & Micci, M. A. (2020). Non-Invasive Transcranial Nano-Pulsed Laser Therapy Ameliorates Cognitive Function and Prevents Aberrant Migration of Neural Progenitor Cells in the Hippocampus of Rats Subjected to Traumatic Brain Injury. Journal of neurotrauma, 37(8), 1108-1123. https://doi.org/10.1089/neu.2019.6534