Noninvasive assessment of ocular tissue using confocal Raman spectroscopy

James P. Wicksted, Noel J C Bauer, Roel J. Erckens, Franciscus H M Jongsma, Eefke E. Smit-Kepel, Massoud Motamedi, Wayne F. March

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

We have developed a confocal Raman spectroscopy system in order to noninvasively characterize ocular tissue with both an in vitro and in vivo capability. This systems consists of a long working distance optical probe, which focuses the incident laser light onto the tissue and collects the backscattered Raman signal, a single grating spectrometer with CCD camera, and an optical fiber which couples the optical probe to the spectrometer. Essential to the confocal design is that the sample volume is limited, preventing detection of interference signals and fluorescence from adjacent ocular tissues. This sample volume is adjustable by changing the diameter of the collection fiber which acts as the pinhole in the system. Potential applications of this technique such as assessing corneal hydration and quantifying pharmacokinetic drug transport across the cornea will be discussed.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages112-122
Number of pages11
Volume3608
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 Biomedical Applications of Raman Spectroscopy - San Jose, Ca, USA
Duration: Jan 25 1999Jan 26 1999

Other

OtherProceedings of the 1999 Biomedical Applications of Raman Spectroscopy
CitySan Jose, Ca, USA
Period1/25/991/26/99

Fingerprint

Raman spectroscopy
Tissue
Spectrometers
spectrometers
Pharmacokinetics
cornea
probes
Diffraction gratings
pinholes
Signal interference
CCD cameras
Hydration
hydration
Optical fibers
drugs
optical fibers
Fluorescence
gratings
interference
fluorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Wicksted, J. P., Bauer, N. J. C., Erckens, R. J., Jongsma, F. H. M., Smit-Kepel, E. E., Motamedi, M., & March, W. F. (1999). Noninvasive assessment of ocular tissue using confocal Raman spectroscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3608, pp. 112-122). Society of Photo-Optical Instrumentation Engineers.

Noninvasive assessment of ocular tissue using confocal Raman spectroscopy. / Wicksted, James P.; Bauer, Noel J C; Erckens, Roel J.; Jongsma, Franciscus H M; Smit-Kepel, Eefke E.; Motamedi, Massoud; March, Wayne F.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608 Society of Photo-Optical Instrumentation Engineers, 1999. p. 112-122.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wicksted, JP, Bauer, NJC, Erckens, RJ, Jongsma, FHM, Smit-Kepel, EE, Motamedi, M & March, WF 1999, Noninvasive assessment of ocular tissue using confocal Raman spectroscopy. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3608, Society of Photo-Optical Instrumentation Engineers, pp. 112-122, Proceedings of the 1999 Biomedical Applications of Raman Spectroscopy, San Jose, Ca, USA, 1/25/99.
Wicksted JP, Bauer NJC, Erckens RJ, Jongsma FHM, Smit-Kepel EE, Motamedi M et al. Noninvasive assessment of ocular tissue using confocal Raman spectroscopy. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608. Society of Photo-Optical Instrumentation Engineers. 1999. p. 112-122
Wicksted, James P. ; Bauer, Noel J C ; Erckens, Roel J. ; Jongsma, Franciscus H M ; Smit-Kepel, Eefke E. ; Motamedi, Massoud ; March, Wayne F. / Noninvasive assessment of ocular tissue using confocal Raman spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3608 Society of Photo-Optical Instrumentation Engineers, 1999. pp. 112-122
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