Noninvasive bioluminescence imaging of dengue virus infection in the brain of A129 mice

Xiao Feng Li, Yong Qiang Deng, Hui Zhao, Qing Ye, Hong Jiang Wang, Shi Hua Li, Shun Ya Zhu, Pei Yong Shi, E. De Qin, Bo Zhang, Cheng Feng Qin

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinant DENV that stably expressed Renilla luciferase (Rluc-DENV), we used bioluminescence for imaging of DENV infection in the brain of A129 mice that lacked type I interferon receptors. Upon intracranial inoculation with Rluc-DENV, A129 mice developed typical neurological symptoms and rapidly succumbed to viral infection. Real-time bioluminescence intensity analysis revealed the replication kinetics of Rluc-DENV in the brain of A129 mice. Linear regression analyses showed a good correlation between photon flux and viral titers (R 2 = 0.9923). Finally, the bioluminescence model was validated using a known mouse monoclonal antibody, 2A10G6, and the therapeutic effects of this neutralizing antibody were readily monitored by live imaging in the same animal. The noninvasive bioluminescence imaging of DENV infection as described here shows distinct advantages over traditional animal models and provides a powerful tool for potential antiviral or vaccine assays against DENV infection in vivo.

Original languageEnglish (US)
Pages (from-to)4589-4596
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume97
Issue number10
DOIs
StatePublished - May 1 2013
Externally publishedYes

Keywords

  • 2A10G6
  • A129 mice
  • Bioluminescence imaging
  • Dengue virus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Li, X. F., Deng, Y. Q., Zhao, H., Ye, Q., Wang, H. J., Li, S. H., Zhu, S. Y., Shi, P. Y., Qin, E. D., Zhang, B., & Qin, C. F. (2013). Noninvasive bioluminescence imaging of dengue virus infection in the brain of A129 mice. Applied Microbiology and Biotechnology, 97(10), 4589-4596. https://doi.org/10.1007/s00253-013-4799-8