Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer

Gracie Vargas, Kert Edward

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Multiphoton autofluorescence microscopy (MPAM) offers the ability to assess morphometry similar to that of pathologic evaluation as well as biochemical information from endogenous fluorophores which are altered with neoplastic transformation. In this study the spectroscopic properties of normal and neoplastic oral epithelium were evaluated toward the goal of identifying image/spectroscopic based indicators of neoplastic transformation using nonlinear optical microscopy. Results indicated measureable differences between normal, dysplasia, and SCC that could be helpful in delineating between the three conditions. In particular, a blue shift in autofluorescence emission was experienced for dysplasia relative to normal. However, in the case of SCC the epithelial emission experienced a significant red shift relative to both dysplasia and normal and displayed in an additional red peak that was not present in either normal or dysplastic mucosa. Results were consistent with published results for SCC in the single-photon literature. The study demonstrates that multiphoton autofluorescence spectroscopy may reveal features of oral mucosa that can be useful for differentiating normal and neoplastic mucosa. When combined with morphometry provided by MPAM, a potentially powerful technique for imaging of the oral cavity could be developed which provides both morphometric and spectroscopic information.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8588
DOIs
StatePublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XIII
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Fingerprint

Optical microscopy
Microscopy
cancer
microscopy
Microscopic examination
Mucous Membrane
Neoplasms
epithelium
Fluorophores
Mouth Mucosa
Photons
blue shift
red shift
Mouth
Spectrum Analysis
Epithelium
Spectroscopy
Imaging techniques
cavities
evaluation

Keywords

  • Multiphoton microscopy
  • Oral precancer
  • Second harmonic generation microscopy
  • Spectroscopy
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Vargas, G., & Edward, K. (2013). Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8588). [85881U] https://doi.org/10.1117/12.2005239

Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer. / Vargas, Gracie; Edward, Kert.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8588 2013. 85881U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Vargas, G & Edward, K 2013, Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8588, 85881U, Multiphoton Microscopy in the Biomedical Sciences XIII, San Francisco, CA, United States, 2/3/13. https://doi.org/10.1117/12.2005239
Vargas G, Edward K. Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8588. 2013. 85881U https://doi.org/10.1117/12.2005239
Vargas, Gracie ; Edward, Kert. / Nonlinear optical microscopy and microspectroscopy of oral precancers and early cancer. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8588 2013.
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