Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow

Yunfei Cai, Nicolas Biais, Gregory Giannone, Monica Tanase, Guoying Jiang, Jake M. Hofman, Chris H. Wiggins, Pascal Silberzan, Axel Buguin, Benoit Ladoux, Michael Sheetz

Research output: Contribution to journalArticle

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Abstract

Nonmuscle myosin IIA (NMM-IIA) is involved in the formation of focal adhesions and neurite retraction. However, the role of NMM-IIA in these functions remains largely unknown. Using RNA interference as a tool to decrease NMM-IIA expression, we have found that NMM-IIA is the major myosin involved in traction force generation and retrograde F-actin flow in mouse embryonic fibroblast cells. Quantitative analyses revealed that ∼60% of traction force on fibronectin-coated surfaces is contributed by NMM-IIA and ∼30% by NMM-IIB. The retrograde F-actin flow decreased dramatically in NMM-IIA-depleted cells, but seemed unaffected by NMM-IIB deletion. In addition, we found that depletion of NMM-IIA caused cells to spread at a higher rate and to a greater area on fibronectin substrates during the early spreading period, whereas deletion of NMM-IIB appeared to have no effect on spreading. The distribution of NMM-IIA was concentrated on the dorsal surface and approached the ventral surface in the periphery, whereas NMM-IIB was primarily concentrated around the nucleus and to a lesser extent at the ventral surface in cell periphery. Our results suggest that NMM-IIA is involved in generating a coherent cytoplasmic contractile force from one side of the cell to the other through the cross-linking and the contraction of dorsal actin filaments.

Original languageEnglish (US)
Pages (from-to)3907-3920
Number of pages14
JournalBiophysical Journal
Volume91
Issue number10
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

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Nonmuscle Myosin Type IIA
Actins
Traction
Fibronectins
Focal Adhesions
Neurites
Myosins
RNA Interference
Actin Cytoskeleton
Fibroblasts

ASJC Scopus subject areas

  • Biophysics

Cite this

Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow. / Cai, Yunfei; Biais, Nicolas; Giannone, Gregory; Tanase, Monica; Jiang, Guoying; Hofman, Jake M.; Wiggins, Chris H.; Silberzan, Pascal; Buguin, Axel; Ladoux, Benoit; Sheetz, Michael.

In: Biophysical Journal, Vol. 91, No. 10, 01.01.2006, p. 3907-3920.

Research output: Contribution to journalArticle

Cai, Y, Biais, N, Giannone, G, Tanase, M, Jiang, G, Hofman, JM, Wiggins, CH, Silberzan, P, Buguin, A, Ladoux, B & Sheetz, M 2006, 'Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow', Biophysical Journal, vol. 91, no. 10, pp. 3907-3920. https://doi.org/10.1529/biophysj.106.084806
Cai, Yunfei ; Biais, Nicolas ; Giannone, Gregory ; Tanase, Monica ; Jiang, Guoying ; Hofman, Jake M. ; Wiggins, Chris H. ; Silberzan, Pascal ; Buguin, Axel ; Ladoux, Benoit ; Sheetz, Michael. / Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow. In: Biophysical Journal. 2006 ; Vol. 91, No. 10. pp. 3907-3920.
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