Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis

Robert V. Intine, Miroslav Dundr, Alex Vassilev, Elena Schwartz, Yingmin Zhao, Yingxin Zhao, Melvin L. Depamphilis, Richard J. Maraia

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

La is a RNA-binding protein implicated in multiple pathways related to the production of tRNAs, ribosomal proteins, and other components of the translational machinery (D. J. Kenan and J. D. Keene, Nat. Struct. Mol. Biol. 11:303-305, 2004). While most La is phosphorylated and resides in the nucleoplasm, a fraction is in the nucleolus, the site of ribosome production, although the determinants of this localization are incompletely known. In addition to its conserved N-terminal domain, human La harbors a C-terminal domain that contains an atypical RNA recognition motif and a short basic motif (SBM) adjacent to phosphoserine-366. We report that nonphosphorylated La (npLa) is concentrated in nucleolar sites that correspond to the dense fibrillar component that harbors nascent pol I transcripts as well as fibrillarin and nucleolin, which function in early phases of rRNA maturation. Affinity purification and native immunoprecipitation of La and fluorescence resonance energy transfer in the nucleolus reveal close association with nucleolin. Moreover, La lacking the SBM does not localize to nucleoli. Lastly, La exhibits SBM-dependent, phosphorylation-sensitive interaction with nucleolin in a yeast two-hybrid assay. The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis.

Original languageEnglish (US)
Pages (from-to)10894-10904
Number of pages11
JournalMolecular and Cellular Biology
Volume24
Issue number24
DOIs
StatePublished - Dec 2004
Externally publishedYes

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Antigens
Ribosomes
Phosphoserine
Fluorescence Resonance Energy Transfer
Two-Hybrid System Techniques
RNA-Binding Proteins
Ribosomal Proteins
Transfer RNA
Immunoprecipitation
Phosphorylation
nucleolin
RNA Recognition Motif
fibrillarin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. / Intine, Robert V.; Dundr, Miroslav; Vassilev, Alex; Schwartz, Elena; Zhao, Yingmin; Zhao, Yingxin; Depamphilis, Melvin L.; Maraia, Richard J.

In: Molecular and Cellular Biology, Vol. 24, No. 24, 12.2004, p. 10894-10904.

Research output: Contribution to journalArticle

Intine, RV, Dundr, M, Vassilev, A, Schwartz, E, Zhao, Y, Zhao, Y, Depamphilis, ML & Maraia, RJ 2004, 'Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis', Molecular and Cellular Biology, vol. 24, no. 24, pp. 10894-10904. https://doi.org/10.1128/MCB.24.24.10894-10904.2004
Intine, Robert V. ; Dundr, Miroslav ; Vassilev, Alex ; Schwartz, Elena ; Zhao, Yingmin ; Zhao, Yingxin ; Depamphilis, Melvin L. ; Maraia, Richard J. / Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 24. pp. 10894-10904.
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