Normobaric hyperoximia increases hypoxia-induced cerebral injury: DTI study in rats

K. H. Bockhorst, P. A. Narayana, J. Dulin, R. Liu, H. C. Rea, K. Hahn, J. Wosik, J. R. Perez-Polo

    Research output: Contribution to journalArticle

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    Abstract

    Perinatal hypoxia affects normal neurological development and can lead to motor, behavioral and cognitive deficits. A common acute treatment for perinatal hypoxia is oxygen resuscitation (hyperoximia), a controversial treatment. Magnetic resonance imaging (MRI), including diffusion tensor imaging (DTI), was performed in a P7 rat model of perinatal hypoxia to determine the effect of hyperoximia. These studies were performed on two groups of animals: 1) animals which were subjected to ischemia followed by hypoxia (HI), and 2) HI followed by hyperoximic treatment (HHI). Lesion volumes on high resolution MRI and DTI derived measures, fractional anisotropy (FA), mean diffusivity (MD), and axial and radial diffusivities (λl and λt, respectively) were measured in vivo one day, one week, and three weeks after injury. Most significant differences in the MRI and DTI measures were found at three weeks after injury. Specifically, three weeks after HHI injury resulted in significantly larger hyperintense lesion volumes (95.26 ± 50.42 mm 3) compared to HI (22.25 ± 17.62 mm3). The radial diffusivity λt of the genu of corpus callosum was significantly larger in HHI (681 ± 330 × 10-6 mm 2/sec) than in HI (486 ± 96 × 10-6 mm 2/sec). Over all, most significant differences in all the DTI metrics (FA, MD, λt, λl) at all time points were found in the corpus callosum. Our results suggest that treatment of perinatal hypoxia with normobaric oxygen does not ameliorate, but exacerbates damage.

    Original languageEnglish (US)
    Pages (from-to)1146-1156
    Number of pages11
    JournalJournal of Neuroscience Research
    Volume88
    Issue number5
    DOIs
    StatePublished - Apr 2010

    Fingerprint

    Brain Hypoxia
    Diffusion Tensor Imaging
    Wounds and Injuries
    Corpus Callosum
    Anisotropy
    Magnetic Resonance Imaging
    Oxygen
    Hypoxia
    Therapeutics
    Resuscitation
    Ischemia

    Keywords

    • Diffusivity
    • Hyperoxia
    • Ischemia
    • MRI
    • Rodents

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience

    Cite this

    Bockhorst, K. H., Narayana, P. A., Dulin, J., Liu, R., Rea, H. C., Hahn, K., ... Perez-Polo, J. R. (2010). Normobaric hyperoximia increases hypoxia-induced cerebral injury: DTI study in rats. Journal of Neuroscience Research, 88(5), 1146-1156. https://doi.org/10.1002/jnr.22273

    Normobaric hyperoximia increases hypoxia-induced cerebral injury : DTI study in rats. / Bockhorst, K. H.; Narayana, P. A.; Dulin, J.; Liu, R.; Rea, H. C.; Hahn, K.; Wosik, J.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 88, No. 5, 04.2010, p. 1146-1156.

    Research output: Contribution to journalArticle

    Bockhorst, KH, Narayana, PA, Dulin, J, Liu, R, Rea, HC, Hahn, K, Wosik, J & Perez-Polo, JR 2010, 'Normobaric hyperoximia increases hypoxia-induced cerebral injury: DTI study in rats', Journal of Neuroscience Research, vol. 88, no. 5, pp. 1146-1156. https://doi.org/10.1002/jnr.22273
    Bockhorst, K. H. ; Narayana, P. A. ; Dulin, J. ; Liu, R. ; Rea, H. C. ; Hahn, K. ; Wosik, J. ; Perez-Polo, J. R. / Normobaric hyperoximia increases hypoxia-induced cerebral injury : DTI study in rats. In: Journal of Neuroscience Research. 2010 ; Vol. 88, No. 5. pp. 1146-1156.
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