Normobaric hyperoximia increases hypoxia-induced cerebral injury: DTI study in rats

K. H. Bockhorst, P. A. Narayana, J. Dulin, R. Liu, H. C. Rea, K. Hahn, J. Wosik, J. R. Perez-Polo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Perinatal hypoxia affects normal neurological development and can lead to motor, behavioral and cognitive deficits. A common acute treatment for perinatal hypoxia is oxygen resuscitation (hyperoximia), a controversial treatment. Magnetic resonance imaging (MRI), including diffusion tensor imaging (DTI), was performed in a P7 rat model of perinatal hypoxia to determine the effect of hyperoximia. These studies were performed on two groups of animals: 1) animals which were subjected to ischemia followed by hypoxia (HI), and 2) HI followed by hyperoximic treatment (HHI). Lesion volumes on high resolution MRI and DTI derived measures, fractional anisotropy (FA), mean diffusivity (MD), and axial and radial diffusivities (λl and λt, respectively) were measured in vivo one day, one week, and three weeks after injury. Most significant differences in the MRI and DTI measures were found at three weeks after injury. Specifically, three weeks after HHI injury resulted in significantly larger hyperintense lesion volumes (95.26 ± 50.42 mm 3) compared to HI (22.25 ± 17.62 mm3). The radial diffusivity λt of the genu of corpus callosum was significantly larger in HHI (681 ± 330 × 10-6 mm 2/sec) than in HI (486 ± 96 × 10-6 mm 2/sec). Over all, most significant differences in all the DTI metrics (FA, MD, λt, λl) at all time points were found in the corpus callosum. Our results suggest that treatment of perinatal hypoxia with normobaric oxygen does not ameliorate, but exacerbates damage.

Original languageEnglish (US)
Pages (from-to)1146-1156
Number of pages11
JournalJournal of Neuroscience Research
Volume88
Issue number5
DOIs
StatePublished - Apr 2010
Externally publishedYes

Keywords

  • Diffusivity
  • Hyperoxia
  • Ischemia
  • MRI
  • Rodents

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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