Novel activation of γ-interferon in nonimmune cells during human cytomegalovirus replication

Istvan Boldogh, T. K. Bui, P. Szaniszlo, W. A. Bresnahan, T. Albrecht, T. K. Hughes

Research output: Contribution to journalArticle

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Abstract

This in the first study documenting the induction of γ-interferon (IFN- γ) in human embryonic fibroblasts during human cytomegalovirus (HCMV) replication. Infection of cells with HCMV resulted in the consistent production of IFN-γ RNA, as determined by RT-PCR and Northern blot analysis. Western blot analysis of cell lysates and immunoprecipitates from the cultural fluids of infected cells demonstrated the presence of IFN-γ at the protein level. Induction of IFN-γ required infectious HCMV, since high-dose ultraviolet inactivation of the virus stock eliminated IFN-γ production. Further, IFN-γ induction appears to be a late event in the virus replication cycle, since inhibition of HCMV DNA synthesis (e.g., phosphonoacetic acid) blocked the increase in IFN-γ. Soluble factor(s) released from HCMV-infected cells apparently did not contribute to the induction of IFN-γ, since virus stocks from which virus had been removed by sedimentation did not induce production of IFN-γ. The appearance of IFN-γ at late stages of HCMV infection and its elimination in the presence of an inhibitor (Actinomycin D) of RNA synthesis indicate a true transcriptional induction of this lymphokine at the RNA and protein levels. The significance of IFN-γ production with regard to the replication and pathogenesis of HCMV in vitro and in vivo will require further investigation.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume215
Issue number1
StatePublished - 1997

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Cytomegalovirus
Viruses
Interferons
Chemical activation
Phosphonoacetic Acid
RNA
Lymphokines
Dactinomycin
Fibroblasts
Sedimentation
Proteins
Cells
Virus Inactivation
Fluids
DNA
Cytomegalovirus Infections
Virus Replication
Northern Blotting
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Novel activation of γ-interferon in nonimmune cells during human cytomegalovirus replication. / Boldogh, Istvan; Bui, T. K.; Szaniszlo, P.; Bresnahan, W. A.; Albrecht, T.; Hughes, T. K.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 215, No. 1, 1997, p. 66-73.

Research output: Contribution to journalArticle

Boldogh, Istvan ; Bui, T. K. ; Szaniszlo, P. ; Bresnahan, W. A. ; Albrecht, T. ; Hughes, T. K. / Novel activation of γ-interferon in nonimmune cells during human cytomegalovirus replication. In: Proceedings of the Society for Experimental Biology and Medicine. 1997 ; Vol. 215, No. 1. pp. 66-73.
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