Novel expression and regulation of gastrin gene in human ovarian cancer cell line, SW626

Hong Jin Kim, B. Mark Evers, Nitesh A. Banker, George H. Greeley, Mark R. Hellmich, James C. Thompson, Courtney M. Townsend

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Gastrin-secreting tumors have been identified in ectopic locations including the ovary; the mechanisms regulating gastrin gene expression, its distribution, and signaling pathways in these ectopic tissues are not known. The purpose of our present study was to determine: (1) whether the gastrin gene and peptide could be detected in ovarian cancer cell lines, (2) if functional gastrin releasing peptide receptors (GRP-R) are present, and (3) whether gastrin gene expression is altered by GRP. Five ovarian cancer cell lines (SW626, OVCA 420, OVCA 429, OVCA 432, and OVCA 433) were analyzed. We identified gastrin gene and peptide expression in the SW626 cell line but not in the OVCA lines. SW626 cells express a functional GRP-R that is correctly coupled to the Ca2+ signaling pathway. Treatment of SW626 cells with bombesin, the amphibian equivalent of GRP, inhibited expression of the gastrin gene in a time- and dose-dependent fashion. The SW626 ovarian cancer cell line will provide a useful model to further define regulation and expression of both the gastrin gene and peptide in ectopic (nongastrointestinal) tissues.

Original languageEnglish (US)
Pages (from-to)1465-1473
Number of pages9
JournalDigestive Diseases and Sciences
Volume43
Issue number7
DOIs
StatePublished - Aug 13 1998

Keywords

  • Gastrin
  • Gastrin releasing peptide
  • Gene expression
  • Ovarian cancer

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Novel expression and regulation of gastrin gene in human ovarian cancer cell line, SW626'. Together they form a unique fingerprint.

  • Cite this