Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome

Dmitri Churikov, Joseph Siino, Maria Svetlova, Kangling Zhang, Arunas Gineitis, E. Morton Bradbury, Andrei Zalensky

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Testis-specific histones are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histones by protamines to epigenetic control of gene transcription. Several testis histone variants were characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (TH2B-175) located at Xq22.2, which encodes a highly divergent H2B variant. The TH2B-175 gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that TH2B-175 evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged TH2B-175 targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, TH2B-175 may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1].

Original languageEnglish (US)
Pages (from-to)745-756
Number of pages12
JournalGenomics
Volume84
Issue number4
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

X-Linked Genes
Histones
Testis
Telomere
Genes
Protamines
Spermatogenesis
Southern Blotting
Cricetulus
Epigenomics
Introns
Primates
Spermatozoa
Rodentia
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Gene Expression
  • Gene structure
  • H2B
  • Histone evolution
  • Histones
  • Telomere
  • Testis

ASJC Scopus subject areas

  • Genetics

Cite this

Churikov, D., Siino, J., Svetlova, M., Zhang, K., Gineitis, A., Morton Bradbury, E., & Zalensky, A. (2004). Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome. Genomics, 84(4), 745-756. https://doi.org/10.1016/j.ygeno.2004.06.001

Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome. / Churikov, Dmitri; Siino, Joseph; Svetlova, Maria; Zhang, Kangling; Gineitis, Arunas; Morton Bradbury, E.; Zalensky, Andrei.

In: Genomics, Vol. 84, No. 4, 10.2004, p. 745-756.

Research output: Contribution to journalArticle

Churikov, D, Siino, J, Svetlova, M, Zhang, K, Gineitis, A, Morton Bradbury, E & Zalensky, A 2004, 'Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome', Genomics, vol. 84, no. 4, pp. 745-756. https://doi.org/10.1016/j.ygeno.2004.06.001
Churikov, Dmitri ; Siino, Joseph ; Svetlova, Maria ; Zhang, Kangling ; Gineitis, Arunas ; Morton Bradbury, E. ; Zalensky, Andrei. / Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome. In: Genomics. 2004 ; Vol. 84, No. 4. pp. 745-756.
@article{952b02e20fec4b5a84ad8ed8e54b1c80,
title = "Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome",
abstract = "Testis-specific histones are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histones by protamines to epigenetic control of gene transcription. Several testis histone variants were characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (TH2B-175) located at Xq22.2, which encodes a highly divergent H2B variant. The TH2B-175 gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that TH2B-175 evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged TH2B-175 targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, TH2B-175 may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1].",
keywords = "Gene Expression, Gene structure, H2B, Histone evolution, Histones, Telomere, Testis",
author = "Dmitri Churikov and Joseph Siino and Maria Svetlova and Kangling Zhang and Arunas Gineitis and {Morton Bradbury}, E. and Andrei Zalensky",
year = "2004",
month = "10",
doi = "10.1016/j.ygeno.2004.06.001",
language = "English (US)",
volume = "84",
pages = "745--756",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome

AU - Churikov, Dmitri

AU - Siino, Joseph

AU - Svetlova, Maria

AU - Zhang, Kangling

AU - Gineitis, Arunas

AU - Morton Bradbury, E.

AU - Zalensky, Andrei

PY - 2004/10

Y1 - 2004/10

N2 - Testis-specific histones are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histones by protamines to epigenetic control of gene transcription. Several testis histone variants were characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (TH2B-175) located at Xq22.2, which encodes a highly divergent H2B variant. The TH2B-175 gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that TH2B-175 evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged TH2B-175 targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, TH2B-175 may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1].

AB - Testis-specific histones are synthesized and accumulated at specific stages of mammalian spermatogenesis. Their proposed functions range from facilitation of the replacement of somatic histones by protamines to epigenetic control of gene transcription. Several testis histone variants were characterized in mouse and rat; however, few are known in humans. Here we report the identification and characterization of a novel human histone 2B gene (TH2B-175) located at Xq22.2, which encodes a highly divergent H2B variant. The TH2B-175 gene contains two introns and is transcribed exclusively in testis, where the spliced polyadenylated mRNA was detected. Genomic PCR, Southern blot analysis, and BLAST-based searches indicate that TH2B-175 evolved in the primate lineage or has been lost in rodents. In transfected Chinese hamster cells, GFP-tagged TH2B-175 targeted to large fluorescent bodies that partially colocalize with the interstitial telomeric blocks. Therefore, TH2B-175 may have telomere-associated functions and participate in the telomere-binding complex in the human sperm [1].

KW - Gene Expression

KW - Gene structure

KW - H2B

KW - Histone evolution

KW - Histones

KW - Telomere

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=4744363626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4744363626&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2004.06.001

DO - 10.1016/j.ygeno.2004.06.001

M3 - Article

VL - 84

SP - 745

EP - 756

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 4

ER -