Novel in vitro respiratory models to study lung development, physiology, pathology and toxicology

Joan Nichols, Jean A. Niles, Stephanie P. Vega, Joaquin Cortiella

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Detailed studies of lung pathology in patients during the course of development of acute lung injury or respiratory distress are limited, and in the past information related to lung-specific responses has been derived from the study of lungs from patients who died at autopsy or from animal models. Development of good in vitro human tissue models would help to bridge the gap in our current knowledge of lung responses and provide a better understanding of lung development, physiology and pathology. In vitro models of simple one-cell or two-cell culture systems as well as complex multicellular lung analogs that reproduce defined components of specific human lung responses have already been realized. A benefit of current in vitro lung models is that hypotheses generated from review of data from human or animal disease studies can be tested directly in engineered human tissue models. Results of studies done using simple in vitro lung systems or more complex three-dimensional models have already been used to examine cell-based responses, physiologic functions, pathologic changes and even drug toxicity or drug responses. In the future we will create models with specific genetic profiles to test the importance of single gene products or pathways of significance. Recent development of microfluidics-based models that support high-throughput screening will allow early-stage toxicity testing in human systems and faster development of new and innovative medical products. Model design in the future will also allow for evaluation of multiple organ systems at once, providing a more holistic or whole-body approach to understanding human physiology and responses.

Original languageEnglish (US)
Article numberS7
JournalStem Cell Research and Therapy
Volume4
Issue numberSUPPL.1
DOIs
StatePublished - Dec 20 2013

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Physiology
Pathology
Toxicology
Lung
Toxicity
Animals
Tissue
In Vitro Techniques
Animal Diseases
Microfluidics
Acute Lung Injury
Drug-Related Side Effects and Adverse Reactions
Cell culture
Pharmaceutical Preparations
Autopsy
Screening
Animal Models
Cell Culture Techniques
Genes
Throughput

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)

Cite this

Novel in vitro respiratory models to study lung development, physiology, pathology and toxicology. / Nichols, Joan; Niles, Jean A.; Vega, Stephanie P.; Cortiella, Joaquin.

In: Stem Cell Research and Therapy, Vol. 4, No. SUPPL.1, S7, 20.12.2013.

Research output: Contribution to journalArticle

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