Novel noninvasive breath test method for screening individuals at risk for diabetes

Edgar Dillon, Morteza Janghorbani, James A. Angel, Shanon L. Casperson, James J. Grady, Randall Urban, Elena Volpi, Melinda Sheffield-Moore

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

OBJECTIVE - Diagnosis of pre-diabetes and early-stage diabetes occurs primarily by means of an oral glucose tolerance test (OGTT), which requires invasive blood sampling. The aim of this study was to determine whether differences exist in breath 13CO 2 excretion during a 13C-labeled OGTT between individuals with normal glucose tolerance (NGT) and individuals with pre-diabetes and early-stage diabetes (PDED) and whether these differences correlated with blood glucose kinetics. RESEARCH DESIGN AND METHODS - Blood and breath samples were collected at baseline and every 30 min for a 10-h period after ingestion of 75 g glucose isotopically labeled with 150 mg [U- 13C 6]D-glucose. RESULTS- Age (56± 5 vs. 47 ± 3 years) and BMI (31 ± 2 vs. 31 ± 2 kg/m 2) were not different between individuals with NGT (n = 10) and PDED (n = 7), respectively. Blood glucose concentrations were significantly higher in those with PDED compared with those with NGT from baseline to 4.5 h after glucose ingestion (P ≤ 0.05). Glucose-derived breath 13CO 2 was significantly lower in individuals with PDED compared with those with NGT from 1 to 3.5 h after glucose (P≤ 0.05). Peak breath 13CO 2 abundance occurred at 4.5 and 3.5 h in individuals with PDED and NGT, respectively (36.87 ± 3.15 vs. 41.36 ± 1.56% delta over baseline). CONCLUSIONS - These results suggest that this novel breath test method may assist in recognition of pre-diabetes or early-stage diabetes in at-risk persons without the need for inva-sive blood sampling, thus making it an attractive option for large-scale testing of at-risk popu-lations, such as children.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalDiabetes Care
Volume32
Issue number3
DOIs
StatePublished - Mar 2009

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Breath Tests
Glucose
Glucose Tolerance Test
Blood Glucose
Eating
Research Design

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialized Nursing

Cite this

Dillon, E., Janghorbani, M., Angel, J. A., Casperson, S. L., Grady, J. J., Urban, R., ... Sheffield-Moore, M. (2009). Novel noninvasive breath test method for screening individuals at risk for diabetes. Diabetes Care, 32(3), 430-435. https://doi.org/10.2337/dc08-1578

Novel noninvasive breath test method for screening individuals at risk for diabetes. / Dillon, Edgar; Janghorbani, Morteza; Angel, James A.; Casperson, Shanon L.; Grady, James J.; Urban, Randall; Volpi, Elena; Sheffield-Moore, Melinda.

In: Diabetes Care, Vol. 32, No. 3, 03.2009, p. 430-435.

Research output: Contribution to journalArticle

Dillon, E, Janghorbani, M, Angel, JA, Casperson, SL, Grady, JJ, Urban, R, Volpi, E & Sheffield-Moore, M 2009, 'Novel noninvasive breath test method for screening individuals at risk for diabetes', Diabetes Care, vol. 32, no. 3, pp. 430-435. https://doi.org/10.2337/dc08-1578
Dillon, Edgar ; Janghorbani, Morteza ; Angel, James A. ; Casperson, Shanon L. ; Grady, James J. ; Urban, Randall ; Volpi, Elena ; Sheffield-Moore, Melinda. / Novel noninvasive breath test method for screening individuals at risk for diabetes. In: Diabetes Care. 2009 ; Vol. 32, No. 3. pp. 430-435.
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abstract = "OBJECTIVE - Diagnosis of pre-diabetes and early-stage diabetes occurs primarily by means of an oral glucose tolerance test (OGTT), which requires invasive blood sampling. The aim of this study was to determine whether differences exist in breath 13CO 2 excretion during a 13C-labeled OGTT between individuals with normal glucose tolerance (NGT) and individuals with pre-diabetes and early-stage diabetes (PDED) and whether these differences correlated with blood glucose kinetics. RESEARCH DESIGN AND METHODS - Blood and breath samples were collected at baseline and every 30 min for a 10-h period after ingestion of 75 g glucose isotopically labeled with 150 mg [U- 13C 6]D-glucose. RESULTS- Age (56± 5 vs. 47 ± 3 years) and BMI (31 ± 2 vs. 31 ± 2 kg/m 2) were not different between individuals with NGT (n = 10) and PDED (n = 7), respectively. Blood glucose concentrations were significantly higher in those with PDED compared with those with NGT from baseline to 4.5 h after glucose ingestion (P ≤ 0.05). Glucose-derived breath 13CO 2 was significantly lower in individuals with PDED compared with those with NGT from 1 to 3.5 h after glucose (P≤ 0.05). Peak breath 13CO 2 abundance occurred at 4.5 and 3.5 h in individuals with PDED and NGT, respectively (36.87 ± 3.15 vs. 41.36 ± 1.56{\%} delta over baseline). CONCLUSIONS - These results suggest that this novel breath test method may assist in recognition of pre-diabetes or early-stage diabetes in at-risk persons without the need for inva-sive blood sampling, thus making it an attractive option for large-scale testing of at-risk popu-lations, such as children.",
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AB - OBJECTIVE - Diagnosis of pre-diabetes and early-stage diabetes occurs primarily by means of an oral glucose tolerance test (OGTT), which requires invasive blood sampling. The aim of this study was to determine whether differences exist in breath 13CO 2 excretion during a 13C-labeled OGTT between individuals with normal glucose tolerance (NGT) and individuals with pre-diabetes and early-stage diabetes (PDED) and whether these differences correlated with blood glucose kinetics. RESEARCH DESIGN AND METHODS - Blood and breath samples were collected at baseline and every 30 min for a 10-h period after ingestion of 75 g glucose isotopically labeled with 150 mg [U- 13C 6]D-glucose. RESULTS- Age (56± 5 vs. 47 ± 3 years) and BMI (31 ± 2 vs. 31 ± 2 kg/m 2) were not different between individuals with NGT (n = 10) and PDED (n = 7), respectively. Blood glucose concentrations were significantly higher in those with PDED compared with those with NGT from baseline to 4.5 h after glucose ingestion (P ≤ 0.05). Glucose-derived breath 13CO 2 was significantly lower in individuals with PDED compared with those with NGT from 1 to 3.5 h after glucose (P≤ 0.05). Peak breath 13CO 2 abundance occurred at 4.5 and 3.5 h in individuals with PDED and NGT, respectively (36.87 ± 3.15 vs. 41.36 ± 1.56% delta over baseline). CONCLUSIONS - These results suggest that this novel breath test method may assist in recognition of pre-diabetes or early-stage diabetes in at-risk persons without the need for inva-sive blood sampling, thus making it an attractive option for large-scale testing of at-risk popu-lations, such as children.

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