Novel radiation response genes identified in gene-trapped MCF10A mammary epithelial cells

Jennifer Malone, Robert Ullrich

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have used a gene-trapping strategy to screen human mammary epithelial cells for radiation response genes. Relative mRNA expression levels of five candidate genes in MCF10A cells were analyzed, both with and without exposure to radiation. In all five cases, the trapped genes were significantly down-regulated after radiation treatment. Sequence analysis of the fusion transcripts identified the trapped genes: (1) the human androgen receptor, (2) the uncharacterized DREV1 gene, which has known homology to DNA methyl-transferases, (3) the human creatine kinase gene, (4) the human eukaryotic translation elongation factor 1 beta 2, and (5) the human ribosomal protein L27. All five genes were down-regulated significantly after treatment with varying doses of ionizing radiation (0.10 to 4.0 Gy) and at varying times (2-30 h after treatment). The genes were also analyzed in human fibroblast and lymphoblastoid cell lines to determine whether the radiation response being observed was cell-type specific. The results verified that the observed radiation response was not a cell-type-specific phenomenon, suggesting that the genes play essential roles in the radiation damage control pathways. This study demonstrates the potential of the gene-trap approach for the identification and functional analysis of novel radiation response genes.

Original languageEnglish (US)
Pages (from-to)176-184
Number of pages9
JournalRadiation Research
Volume167
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

genes
breasts
Breast
epithelial cells
Epithelial Cells
Radiation
radiation
Genes
cells
Peptide Elongation Factor 1
creatine
functional analysis
ribosomal proteins
Essential Genes
creatine kinase
gene targeting
Creatine Kinase
Transferases
Ionizing Radiation
ionizing radiation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Novel radiation response genes identified in gene-trapped MCF10A mammary epithelial cells. / Malone, Jennifer; Ullrich, Robert.

In: Radiation Research, Vol. 167, No. 2, 02.2007, p. 176-184.

Research output: Contribution to journalArticle

Malone, Jennifer ; Ullrich, Robert. / Novel radiation response genes identified in gene-trapped MCF10A mammary epithelial cells. In: Radiation Research. 2007 ; Vol. 167, No. 2. pp. 176-184.
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