Novel universal primers establish identity of an enormous number of animal species for forensic application

S. K. Verma, Lalji Singh

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

This study describes a polymerase chain reaction (PCR)-based approach, which without knowing the history of a forensic sample, is able to reveal whether the source of the sample is human or animal and, if animal which of the 221 animal species included in the study, simply by using one set of novel primers to amplify and sequence the PCR amplicons. The primers described in this study universally amplify a specific segment of mitochondrial cytochrome b sequence from a sample of unknown origin and delineate its identity to the level of family, genus and species. Because these primers are universal, this approach can be applied to an enormous number of other species, which are not included in the study, and could be an ultimate solution for the identification of species for forensic application.

Original languageEnglish (US)
Pages (from-to)28-31
Number of pages4
JournalMolecular Ecology Notes
Volume3
Issue number1
DOIs
StatePublished - Mar 2003
Externally publishedYes

Fingerprint

Animals
Polymerase chain reaction
polymerase chain reaction
Polymerase Chain Reaction
animals
Cytochromes b
animal
cytochrome b
sampling
cytochrome
History
history
forensic sciences
animal species
species identification
family

Keywords

  • Forensics
  • Species identification
  • Universal primers
  • Wildlife

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Novel universal primers establish identity of an enormous number of animal species for forensic application. / Verma, S. K.; Singh, Lalji.

In: Molecular Ecology Notes, Vol. 3, No. 1, 03.2003, p. 28-31.

Research output: Contribution to journalArticle

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