Novel urinary fragments from human basement membrane collagen

B. H. Bowman, L. Schneider, D. R. Barnett, A. Kurosky, R. M. Goldblum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Five fragments of collagen have been purified from human urine. The fragments cross-reacted with basement membrane collagen from pig kidney cortices when tested against antiserum to basement membrane collagen, type IV, from murine tumor. The concentrations of these fragments were sufficient for amino acid analysis, immunological analysis, and in one case, preliminary analytical amino acid sequencing. Compositional analysis of the fragments indicated concentrations of hydroxylysine, hydroxyproline, and glycine that are typical of collagen. High values of hexosamines and neutral sugars were also found. An NH 2-terminal sequence of 18 residues was obtained for one fragment of M(r)~6,500. This sequence was unique among proteins reported to date, but was obviously collagen-like. A urinary source of basement membrane collagen fragments will permit molecular characterization of basement membrane in inherited or acquired diseases affecting this structure.

Original languageEnglish (US)
Pages (from-to)9484-9489
Number of pages6
JournalJournal of Biological Chemistry
Volume255
Issue number19
StatePublished - 1980
Externally publishedYes

Fingerprint

Basement Membrane
Collagen
Hydroxylysine
Hexosamines
Amino Acids
Kidney Cortex
Collagen Type IV
Hydroxyproline
Protein Sequence Analysis
Sugars
Glycine
Tumors
Immune Sera
Swine
Urine
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bowman, B. H., Schneider, L., Barnett, D. R., Kurosky, A., & Goldblum, R. M. (1980). Novel urinary fragments from human basement membrane collagen. Journal of Biological Chemistry, 255(19), 9484-9489.

Novel urinary fragments from human basement membrane collagen. / Bowman, B. H.; Schneider, L.; Barnett, D. R.; Kurosky, A.; Goldblum, R. M.

In: Journal of Biological Chemistry, Vol. 255, No. 19, 1980, p. 9484-9489.

Research output: Contribution to journalArticle

Bowman, BH, Schneider, L, Barnett, DR, Kurosky, A & Goldblum, RM 1980, 'Novel urinary fragments from human basement membrane collagen', Journal of Biological Chemistry, vol. 255, no. 19, pp. 9484-9489.
Bowman BH, Schneider L, Barnett DR, Kurosky A, Goldblum RM. Novel urinary fragments from human basement membrane collagen. Journal of Biological Chemistry. 1980;255(19):9484-9489.
Bowman, B. H. ; Schneider, L. ; Barnett, D. R. ; Kurosky, A. ; Goldblum, R. M. / Novel urinary fragments from human basement membrane collagen. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 19. pp. 9484-9489.
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