Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees

3 approved]

Claire L. Jeffries, Gena G. Lawrence, George Golovko, Mojca Kristan, James Orsborne, Kirstin Spence, Eliot Hurn, Janvier Bandibabone, Luciano M. Tantely, Fara N. Raharimalala, Kalil Keita, Denka Camara, Yaya Barry, Francis Wat’senga, Emile Z. Manzambi, Yaw A. Afrane, Abdul R. Mohammed, Tarekegn A. Abeku, Shivanand Hegde, Kamil Khanipov & 6 others Maria Pimenova, Yuriy Fofanov, Sebastien Boyer, Seth R. Irish, Grant L. Hughes, Thomas Walker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations in West Africa. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species across five malaria endemic countries to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species A, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species A. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species A but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.

Original languageEnglish (US)
Article number113
JournalWellcome Open Research
Volume3
DOIs
StatePublished - Jan 1 2018

Fingerprint

Wolbachia
Anopheles
Africa South of the Sahara
Malaria
Anopheles gambiae
Bacteria
Culicidae
Pathogens
Guinea
Madagascar
Democratic Republic of the Congo
Multilocus Sequence Typing
Ghana
Western Africa
Infectious Disease Transmission
Uganda
Plasmodium
Microbiota
Population Dynamics
Genes

Keywords

  • Anopheles
  • Asaia
  • Endosymbionts
  • Malaria
  • Mosquitoes
  • Wolbachia

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees : 3 approved]. / Jeffries, Claire L.; Lawrence, Gena G.; Golovko, George; Kristan, Mojca; Orsborne, James; Spence, Kirstin; Hurn, Eliot; Bandibabone, Janvier; Tantely, Luciano M.; Raharimalala, Fara N.; Keita, Kalil; Camara, Denka; Barry, Yaya; Wat’senga, Francis; Manzambi, Emile Z.; Afrane, Yaw A.; Mohammed, Abdul R.; Abeku, Tarekegn A.; Hegde, Shivanand; Khanipov, Kamil; Pimenova, Maria; Fofanov, Yuriy; Boyer, Sebastien; Irish, Seth R.; Hughes, Grant L.; Walker, Thomas.

In: Wellcome Open Research, Vol. 3, 113, 01.01.2018.

Research output: Contribution to journalArticle

Jeffries, CL, Lawrence, GG, Golovko, G, Kristan, M, Orsborne, J, Spence, K, Hurn, E, Bandibabone, J, Tantely, LM, Raharimalala, FN, Keita, K, Camara, D, Barry, Y, Wat’senga, F, Manzambi, EZ, Afrane, YA, Mohammed, AR, Abeku, TA, Hegde, S, Khanipov, K, Pimenova, M, Fofanov, Y, Boyer, S, Irish, SR, Hughes, GL & Walker, T 2018, 'Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees: 3 approved]', Wellcome Open Research, vol. 3, 113. https://doi.org/10.12688/wellcomeopenres.14765.2
Jeffries, Claire L. ; Lawrence, Gena G. ; Golovko, George ; Kristan, Mojca ; Orsborne, James ; Spence, Kirstin ; Hurn, Eliot ; Bandibabone, Janvier ; Tantely, Luciano M. ; Raharimalala, Fara N. ; Keita, Kalil ; Camara, Denka ; Barry, Yaya ; Wat’senga, Francis ; Manzambi, Emile Z. ; Afrane, Yaw A. ; Mohammed, Abdul R. ; Abeku, Tarekegn A. ; Hegde, Shivanand ; Khanipov, Kamil ; Pimenova, Maria ; Fofanov, Yuriy ; Boyer, Sebastien ; Irish, Seth R. ; Hughes, Grant L. ; Walker, Thomas. / Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees : 3 approved]. In: Wellcome Open Research. 2018 ; Vol. 3.
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abstract = "Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations in West Africa. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species across five malaria endemic countries to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species A, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species A. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species A but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.",
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T1 - Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees

T2 - 3 approved]

AU - Jeffries, Claire L.

AU - Lawrence, Gena G.

AU - Golovko, George

AU - Kristan, Mojca

AU - Orsborne, James

AU - Spence, Kirstin

AU - Hurn, Eliot

AU - Bandibabone, Janvier

AU - Tantely, Luciano M.

AU - Raharimalala, Fara N.

AU - Keita, Kalil

AU - Camara, Denka

AU - Barry, Yaya

AU - Wat’senga, Francis

AU - Manzambi, Emile Z.

AU - Afrane, Yaw A.

AU - Mohammed, Abdul R.

AU - Abeku, Tarekegn A.

AU - Hegde, Shivanand

AU - Khanipov, Kamil

AU - Pimenova, Maria

AU - Fofanov, Yuriy

AU - Boyer, Sebastien

AU - Irish, Seth R.

AU - Hughes, Grant L.

AU - Walker, Thomas

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations in West Africa. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species across five malaria endemic countries to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species A, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species A. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species A but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.

AB - Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations in West Africa. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species across five malaria endemic countries to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species A, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species A. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species A but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.

KW - Anopheles

KW - Asaia

KW - Endosymbionts

KW - Malaria

KW - Mosquitoes

KW - Wolbachia

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