Novel zinc finger proteins that interact with the mouse γF-crystallin promoter and are expressed in the sclerotome during early somitogenesis

Qiurong Liu, Fouad Shalaby, Mira C. Puri, Shao-Jun Tang, Martin L. Breitman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Lens-specific expression of the mouse γF-crystallin gene is determined, at least in part, by a 23-bp DNA element, the γF-1-binding motif, located in the promoter region of the gene. To characterize the transcription factors that regulate γF-crystallin gene expression through this element, we have isolated three chicken cDNAs that encode proteins capable of binding specifically to the γF-1-binding motif. These three cDNAs represent differential splicing products from a single gene, γFBP. The protein isoforms encoded by two of these cDNAs differ in their ability to modulate the activity of promoters containing the γF-1-binding motif. Among them, γFBP-B functions as a transcriptional repressor in lens cells, and it's expression is developmentally regulated during lens development, suggesting a role for this isoform in the spatial regulation of γF-crystallin gene expression. We also show that expression of the different mRNA transcripts are differentially regulated in various tissues. Furthermore, γFBP transcripts are highly expressed in presomitic mesoderm and then over the entire epithelial somite. During somitic differentiation, γFBP expression becomes restricted to the sclerotome. These expression patterns suggest a regulatory role for the γFBP isoforms in sclerotome specification and/or differentiation.

Original languageEnglish (US)
Pages (from-to)165-177
Number of pages13
JournalDevelopmental Biology
Volume165
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Crystallins
Zinc Fingers
Lenses
Protein Isoforms
Complementary DNA
Genes
Gene Expression
Somites
Proteins
Mesoderm
Genetic Promoter Regions
Protein Binding
Chickens
Transcription Factors
Messenger RNA
DNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Novel zinc finger proteins that interact with the mouse γF-crystallin promoter and are expressed in the sclerotome during early somitogenesis. / Liu, Qiurong; Shalaby, Fouad; Puri, Mira C.; Tang, Shao-Jun; Breitman, Martin L.

In: Developmental Biology, Vol. 165, No. 1, 1994, p. 165-177.

Research output: Contribution to journalArticle

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abstract = "Lens-specific expression of the mouse γF-crystallin gene is determined, at least in part, by a 23-bp DNA element, the γF-1-binding motif, located in the promoter region of the gene. To characterize the transcription factors that regulate γF-crystallin gene expression through this element, we have isolated three chicken cDNAs that encode proteins capable of binding specifically to the γF-1-binding motif. These three cDNAs represent differential splicing products from a single gene, γFBP. The protein isoforms encoded by two of these cDNAs differ in their ability to modulate the activity of promoters containing the γF-1-binding motif. Among them, γFBP-B functions as a transcriptional repressor in lens cells, and it's expression is developmentally regulated during lens development, suggesting a role for this isoform in the spatial regulation of γF-crystallin gene expression. We also show that expression of the different mRNA transcripts are differentially regulated in various tissues. Furthermore, γFBP transcripts are highly expressed in presomitic mesoderm and then over the entire epithelial somite. During somitic differentiation, γFBP expression becomes restricted to the sclerotome. These expression patterns suggest a regulatory role for the γFBP isoforms in sclerotome specification and/or differentiation.",
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AU - Tang, Shao-Jun

AU - Breitman, Martin L.

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