Abstract
Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8274-8278 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 80 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 2006 |
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ASJC Scopus subject areas
- Immunology
Cite this
NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture. / Won, Sungyong; Ikegami, Tetsuro; Peters, C. J.; Makino, Shinji.
In: Journal of Virology, Vol. 80, No. 16, 08.2006, p. 8274-8278.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture
AU - Won, Sungyong
AU - Ikegami, Tetsuro
AU - Peters, C. J.
AU - Makino, Shinji
PY - 2006/8
Y1 - 2006/8
N2 - Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins.
AB - Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins.
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UR - http://www.scopus.com/inward/citedby.url?scp=33746784160&partnerID=8YFLogxK
U2 - 10.1128/JVI.00476-06
DO - 10.1128/JVI.00476-06
M3 - Article
VL - 80
SP - 8274
EP - 8278
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 16
ER -