NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture

Sungyong Won, Tetsuro Ikegami, C. J. Peters, Shinji Makino

Research output: Contribution to journalArticle

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Abstract

Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins.

Original languageEnglish (US)
Pages (from-to)8274-8278
Number of pages5
JournalJournal of Virology
Volume80
Issue number16
DOIs
StatePublished - Aug 2006

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Rift Valley fever virus
virus replication
cell culture
Cell Culture Techniques
protein synthesis
viruses
Viruses
Proteins
proteins
mutants
Vero Cells
cells
mutation
kinetics
assays
Mutation
Growth
genes
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture. / Won, Sungyong; Ikegami, Tetsuro; Peters, C. J.; Makino, Shinji.

In: Journal of Virology, Vol. 80, No. 16, 08.2006, p. 8274-8278.

Research output: Contribution to journalArticle

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