Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

Anthony Okorodudu, W. Clark Lambert, Muriel W. Lambert

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.

Original languageEnglish (US)
Pages (from-to)576-584
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number2
DOIs
StatePublished - Sep 30 1982
Externally publishedYes

Fingerprint

Xeroderma Pigmentosum
Deoxyribonucleases
Chromatin
Endonucleases
Exonucleases
Deoxyribonuclease I
Isoelectric Focusing
Cells
Cell Line
DNA
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells. / Okorodudu, Anthony; Lambert, W. Clark; Lambert, Muriel W.

In: Biochemical and Biophysical Research Communications, Vol. 108, No. 2, 30.09.1982, p. 576-584.

Research output: Contribution to journalArticle

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