Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

Anthony O. Okorodudu, W. Clark Lambert, Muriel W. Lambert

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.

Original languageEnglish (US)
Pages (from-to)576-584
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number2
DOIs
StatePublished - Sep 30 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells'. Together they form a unique fingerprint.

  • Cite this