Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

Anthony O. Okorodudu; W. Clark Lambert; Muriel W. Lambert

doi:10.1016/0006-291X(82)90867-1

Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

Anthony O. Okorodudu
, W. Clark Lambert
, Muriel W. Lambert

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.

Original language	English (US)
Pages (from-to)	576-584
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	108
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(82)90867-1
State	Published - Sep 30 1982
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(82)90867-1

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@article{d1025a9c52d445bbb292ef6823fe5a5d,

title = "Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells",

abstract = "Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.",

author = "Okorodudu, \{Anthony O.\} and Lambert, \{W. Clark\} and Lambert, \{Muriel W.\}",

note = "Funding Information: This work was supported in part by Basil O'Connor Grant \#5-287 from the March of Dimes Birth Defects Foundation, the Dermatology Foundation and the Fourrlation of the UIW.",

year = "1982",

month = sep,

day = "30",

doi = "10.1016/0006-291X(82)90867-1",

language = "English (US)",

volume = "108",

pages = "576--584",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

AU - Okorodudu, Anthony O.

AU - Lambert, W. Clark

AU - Lambert, Muriel W.

N1 - Funding Information: This work was supported in part by Basil O'Connor Grant #5-287 from the March of Dimes Birth Defects Foundation, the Dermatology Foundation and the Fourrlation of the UIW.

PY - 1982/9/30

Y1 - 1982/9/30

N2 - Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.

AB - Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone chromatin-associated and nucleoplasmic proteins of isolated nuclei of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid cells using parallel procedures. In the nucleoplasm of both cell lines, a very similar series of both DNA endo- and exo-nuclease activities were found; in chromatin a series of similar endonuclease but no exonuclease activites were present. Several differences were observed in the xeroderma pigmentosum cells, however, notably a striking increase in DNA endonuclease activity in a chromatin fraction at pI 4.6 against linear duplex DNA and a decrease in a chromatin endonuclease activity focusing at pI 7.8.

UR - https://www.scopus.com/pages/publications/0020457568

UR - https://www.scopus.com/pages/publications/0020457568#tab=citedBy

U2 - 10.1016/0006-291X(82)90867-1

DO - 10.1016/0006-291X(82)90867-1

M3 - Article

C2 - 6216888

AN - SCOPUS:0020457568

SN - 0006-291X

VL - 108

SP - 576

EP - 584

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells

Abstract

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