Nucleic acid-based inhibition of flavivirus infections

David A. Stein, Pei-Yong Shi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The genus Flavivirus in the family Flaviviridae contains many arthropod-transmitted human pathogens, including dengue, yellow fever, Japanese encephalitis, West Nile, St. Louis encephalitis, Murray Valley encephalitis, and tick-borne encephalitis viruses. Treatment options for flaviviral diseases are extremely limited, with no effective drugs yet commercially available. Recent advances in virology, synthetic organic chemistry, and the discovery of RNA interference (RNAi), have provided the basis for advances in the development of antisense-based approaches to address flaviviral infections. Oligomers of various antisense structural types, targeted to different locations in the flaviviral RNA genome, have now been used to successfully suppress viral gene expression and thereby inhibit flavivirus replication. Double-stranded RNA, containing viral sequence and designed to induce the endogenous cellular machinery of RNAi, has also been shown capable of potently interfering with flavivirus production and transmission. These studies provide insights into flaviviral molecular biology and the basis for the development of novel therapeutic approaches. The goal of this review is to summarize the findings of many of the significant reports that have appeared on the topic of antisense-mediated strategies for the development of antiviral therapy for flaviviruses.

Original languageEnglish (US)
Pages (from-to)1385-1395
Number of pages11
JournalFrontiers in Bioscience
Volume13
Issue number4
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Flavivirus Infections
Flavivirus
Nucleic Acids
RNA
RNA Interference
St. Louis Encephalitis
Molecular biology
Double-Stranded RNA
Flaviviridae
Pathogens
Organic Chemistry
Japanese Encephalitis
Tick-Borne Encephalitis Viruses
Yellow Fever
Viruses
Oligomers
Gene expression
Virology
Machinery
Antiviral Agents

Keywords

  • Antisense
  • Antiviral
  • Drug development
  • Flavivirus
  • Review
  • RNAi

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Nucleic acid-based inhibition of flavivirus infections. / Stein, David A.; Shi, Pei-Yong.

In: Frontiers in Bioscience, Vol. 13, No. 4, 2008, p. 1385-1395.

Research output: Contribution to journalArticle

Stein, David A. ; Shi, Pei-Yong. / Nucleic acid-based inhibition of flavivirus infections. In: Frontiers in Bioscience. 2008 ; Vol. 13, No. 4. pp. 1385-1395.
@article{fa6ef10f195e466f935dc86b6e3c3c06,
title = "Nucleic acid-based inhibition of flavivirus infections",
abstract = "The genus Flavivirus in the family Flaviviridae contains many arthropod-transmitted human pathogens, including dengue, yellow fever, Japanese encephalitis, West Nile, St. Louis encephalitis, Murray Valley encephalitis, and tick-borne encephalitis viruses. Treatment options for flaviviral diseases are extremely limited, with no effective drugs yet commercially available. Recent advances in virology, synthetic organic chemistry, and the discovery of RNA interference (RNAi), have provided the basis for advances in the development of antisense-based approaches to address flaviviral infections. Oligomers of various antisense structural types, targeted to different locations in the flaviviral RNA genome, have now been used to successfully suppress viral gene expression and thereby inhibit flavivirus replication. Double-stranded RNA, containing viral sequence and designed to induce the endogenous cellular machinery of RNAi, has also been shown capable of potently interfering with flavivirus production and transmission. These studies provide insights into flaviviral molecular biology and the basis for the development of novel therapeutic approaches. The goal of this review is to summarize the findings of many of the significant reports that have appeared on the topic of antisense-mediated strategies for the development of antiviral therapy for flaviviruses.",
keywords = "Antisense, Antiviral, Drug development, Flavivirus, Review, RNAi",
author = "Stein, {David A.} and Pei-Yong Shi",
year = "2008",
doi = "10.2741/2769",
language = "English (US)",
volume = "13",
pages = "1385--1395",
journal = "Frontiers in Bioscience - Landmark",
issn = "1093-9946",
publisher = "Frontiers in Bioscience",
number = "4",

}

TY - JOUR

T1 - Nucleic acid-based inhibition of flavivirus infections

AU - Stein, David A.

AU - Shi, Pei-Yong

PY - 2008

Y1 - 2008

N2 - The genus Flavivirus in the family Flaviviridae contains many arthropod-transmitted human pathogens, including dengue, yellow fever, Japanese encephalitis, West Nile, St. Louis encephalitis, Murray Valley encephalitis, and tick-borne encephalitis viruses. Treatment options for flaviviral diseases are extremely limited, with no effective drugs yet commercially available. Recent advances in virology, synthetic organic chemistry, and the discovery of RNA interference (RNAi), have provided the basis for advances in the development of antisense-based approaches to address flaviviral infections. Oligomers of various antisense structural types, targeted to different locations in the flaviviral RNA genome, have now been used to successfully suppress viral gene expression and thereby inhibit flavivirus replication. Double-stranded RNA, containing viral sequence and designed to induce the endogenous cellular machinery of RNAi, has also been shown capable of potently interfering with flavivirus production and transmission. These studies provide insights into flaviviral molecular biology and the basis for the development of novel therapeutic approaches. The goal of this review is to summarize the findings of many of the significant reports that have appeared on the topic of antisense-mediated strategies for the development of antiviral therapy for flaviviruses.

AB - The genus Flavivirus in the family Flaviviridae contains many arthropod-transmitted human pathogens, including dengue, yellow fever, Japanese encephalitis, West Nile, St. Louis encephalitis, Murray Valley encephalitis, and tick-borne encephalitis viruses. Treatment options for flaviviral diseases are extremely limited, with no effective drugs yet commercially available. Recent advances in virology, synthetic organic chemistry, and the discovery of RNA interference (RNAi), have provided the basis for advances in the development of antisense-based approaches to address flaviviral infections. Oligomers of various antisense structural types, targeted to different locations in the flaviviral RNA genome, have now been used to successfully suppress viral gene expression and thereby inhibit flavivirus replication. Double-stranded RNA, containing viral sequence and designed to induce the endogenous cellular machinery of RNAi, has also been shown capable of potently interfering with flavivirus production and transmission. These studies provide insights into flaviviral molecular biology and the basis for the development of novel therapeutic approaches. The goal of this review is to summarize the findings of many of the significant reports that have appeared on the topic of antisense-mediated strategies for the development of antiviral therapy for flaviviruses.

KW - Antisense

KW - Antiviral

KW - Drug development

KW - Flavivirus

KW - Review

KW - RNAi

UR - http://www.scopus.com/inward/record.url?scp=38449115143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38449115143&partnerID=8YFLogxK

U2 - 10.2741/2769

DO - 10.2741/2769

M3 - Article

C2 - 17981637

AN - SCOPUS:38449115143

VL - 13

SP - 1385

EP - 1395

JO - Frontiers in Bioscience - Landmark

JF - Frontiers in Bioscience - Landmark

SN - 1093-9946

IS - 4

ER -