Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal

Krishna Narayanan, Chun Jen Chen, Junko Maeda, Shinji Makino

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

For any of the enveloped RNA viruses studied to date, recognition of a specific RNA packaging signal by the virus's nucleocapsid (N) protein is the first step described in the process of viral RNA packaging. In the murine coronavirus a selective interaction between the viral transmembrane envelope protein M and the viral ribonucleoprotein complex, composed of N protein and viral RNA containing a short cis-acting RNA element, the packaging signal, determines the selective RNA packaging into virus particles. In this report we show that expressed coronavirus envelope protein M specifically interacted with coexpressed noncoronavirus RNA transcripts containing the short viral packaging signal in the absence of coronavirus N protein. Furthermore, this M protein-packaging signal interaction led to specific packaging of the packaging signal-containing RNA transcripts into coronavirus-like particles in the absence of N protein. These findings not only highlight a novel RNA packaging mechanism for an enveloped virus, where the specific RNA packaging can occur without the core or N protein, but also point to a new, biologically important general model of precise and selective interaction between transmembrane proteins and specific RNA elements.

Original languageEnglish (US)
Pages (from-to)2922-2927
Number of pages6
JournalJournal of Virology
Volume77
Issue number5
DOIs
StatePublished - Mar 2003

Fingerprint

Viral Envelope Proteins
Virus Assembly
Nucleocapsid
nucleocapsid
Viral RNA
Product Packaging
RNA
packaging
Coronavirinae
proteins
Coronavirus
RNA Viruses
Proteins
messenger RNA
Murine coronavirus
Nucleocapsid Proteins
Ribonucleoproteins
ribonucleoproteins
viruses
transmembrane proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal. / Narayanan, Krishna; Chen, Chun Jen; Maeda, Junko; Makino, Shinji.

In: Journal of Virology, Vol. 77, No. 5, 03.2003, p. 2922-2927.

Research output: Contribution to journalArticle

Narayanan, Krishna ; Chen, Chun Jen ; Maeda, Junko ; Makino, Shinji. / Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal. In: Journal of Virology. 2003 ; Vol. 77, No. 5. pp. 2922-2927.
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