Nucleosome interactions with a human Alu element

Transcriptional repression and effects of template methylation

Ella Englander, Alan P. Wolffe, Bruce H. Howard

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Alu interspersed repetitive elements possess internal RNA polymerase III promoters which are strongly transcribed in vitro, yet these elements are nearly silent in somatic cells. To examine whether repression by chromatin proteins could contribute to the low level of Alu expression, a conserved Alu element from the fourth intron of the human α-fetoprotein gene was reconstituted with purified octamer or tetramer particles. Analysis of reconstitutes revealed that this Alu element directed translational and rotational positioning of octamers as well as tetramers. In vitro transcription experiments with reconstituted templates demonstrated that RNA polymerase III-dependent transcription of the Alu element was profoundly repressed by positioned octamer particles. Furthermore, complete CpG methylation of this template enhanced the capacity of tetramers to repress transcription.

Original languageEnglish (US)
Pages (from-to)19565-19573
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - Sep 15 1993
Externally publishedYes

Fingerprint

Alu Elements
Methylation
Nucleosomes
Transcription
RNA Polymerase III
Fetal Proteins
Interspersed Repetitive Sequences
Introns
Chromatin
Genes
Proteins
Experiments
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nucleosome interactions with a human Alu element : Transcriptional repression and effects of template methylation. / Englander, Ella; Wolffe, Alan P.; Howard, Bruce H.

In: Journal of Biological Chemistry, Vol. 268, No. 26, 15.09.1993, p. 19565-19573.

Research output: Contribution to journalArticle

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