Nucleotide sequence analysis of purH and purD genes from Salmonella typhimurium

Ashok Chopra, Johnny Peterson, Rajendra Prasad

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The purH and purD genes coding for the 5′-phosphoribosyl 5-amino-imidazole-4-carboxamide (AICAR) transformylase and 5′-phosphoribosyl-glycinamide (GAR) synthetase, respectively, were identified on a 4.8 kb EcoRI fragment of chromosomal DNA from Salmonella typhimurium. Nucleotide sequence analysis of the cloned fragment revealed the presence of two large open reading frames (O.R.F.), which were separated by 11 base pairs (bp). Substantial DNA and amino acid sequence homology was noted between the purH and purD genes of S. typhimurium and Escherichia coli. Expression of the Salmonella purD gene in a T7 polymerase/promoter system revealed the presence of a 49 kDa protein band by SDS-PAGE and subsequent autoradiography. The purH gene of Salmonella was not expressed since the 5′ end of this gene was not cloned.

Original languageEnglish (US)
Pages (from-to)351-354
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1090
Issue number3
DOIs
StatePublished - Nov 11 1991

Fingerprint

Salmonella
Salmonella typhimurium
Sequence Analysis
Nucleotides
Genes
Hydroxymethyl and Formyl Transferases
Amino Acid Sequence Homology
DNA
Autoradiography
Base Pairing
Escherichia coli
Open Reading Frames
Polyacrylamide Gel Electrophoresis
Amino Acids
Proteins

Keywords

  • DNA sequencing
  • purH and purD genes
  • T7 polymerase/promoter system

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Nucleotide sequence analysis of purH and purD genes from Salmonella typhimurium. / Chopra, Ashok; Peterson, Johnny; Prasad, Rajendra.

In: BBA - Gene Structure and Expression, Vol. 1090, No. 3, 11.11.1991, p. 351-354.

Research output: Contribution to journalArticle

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N2 - The purH and purD genes coding for the 5′-phosphoribosyl 5-amino-imidazole-4-carboxamide (AICAR) transformylase and 5′-phosphoribosyl-glycinamide (GAR) synthetase, respectively, were identified on a 4.8 kb EcoRI fragment of chromosomal DNA from Salmonella typhimurium. Nucleotide sequence analysis of the cloned fragment revealed the presence of two large open reading frames (O.R.F.), which were separated by 11 base pairs (bp). Substantial DNA and amino acid sequence homology was noted between the purH and purD genes of S. typhimurium and Escherichia coli. Expression of the Salmonella purD gene in a T7 polymerase/promoter system revealed the presence of a 49 kDa protein band by SDS-PAGE and subsequent autoradiography. The purH gene of Salmonella was not expressed since the 5′ end of this gene was not cloned.

AB - The purH and purD genes coding for the 5′-phosphoribosyl 5-amino-imidazole-4-carboxamide (AICAR) transformylase and 5′-phosphoribosyl-glycinamide (GAR) synthetase, respectively, were identified on a 4.8 kb EcoRI fragment of chromosomal DNA from Salmonella typhimurium. Nucleotide sequence analysis of the cloned fragment revealed the presence of two large open reading frames (O.R.F.), which were separated by 11 base pairs (bp). Substantial DNA and amino acid sequence homology was noted between the purH and purD genes of S. typhimurium and Escherichia coli. Expression of the Salmonella purD gene in a T7 polymerase/promoter system revealed the presence of a 49 kDa protein band by SDS-PAGE and subsequent autoradiography. The purH gene of Salmonella was not expressed since the 5′ end of this gene was not cloned.

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