TY - JOUR
T1 - Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype
T2 - Comparative analysis of the full-length genome
AU - Deubel, Vincent
AU - Kinney, Richard M.
AU - Trent, Dennis W.
N1 - Funding Information:
We thank Drs. Vance Vorndam and Gwong Jen Chang for providing the synthesized oligonucleotides, Joyce Grant for technical assistance, Dr. James Strauss for providing the DEN2Sl virus sequence before publication, Gerard Masson for assistance in computer analyses, and Judy Parizek for expert typing. This research was supported in part by the Direction des Recherches Etudes et Techniques 85 34 820 00 470 75 01 (Paris) and by Fellowship 1 F05 TWO 3596 from the International Research and Awards Branch, National Institutes of Health, Bethesda, Maryland.
PY - 1988/7
Y1 - 1988/7
N2 - The sequence of the 5′-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,723 bases in length with a single open reading frame extending from nucleotides 97 to 10,269 encoding 3391 amino acids. The 3′-noncoding extremity presents a stem- and loop-structure and contains a repeated oligonucleotide sequence. Comparisons of the nucleotide sequences of the genomes of dengue 2 viruses of different topotypes reveal 90-95% similarity, with 64-66% similarity evident between dengue viruses of different serotypes. The amino acid sequence of the polyprotein of dengue 2 Jamaica virus shows 97, 68, 50, and 44% similarity with those of other dengue 2, dengue 1, or dengue 4, West Nile, and yellow fever viruses, respectively. Despite amino acid sequence divergence, the hydrophobic profile of the flavivirus proteins is highly conserved. Proteins NS1, NS3, and NS5 are the most conserved. Conserved amino acid stretches present in all flavivirus proteins may be involved in common essential biological functions.
AB - The sequence of the 5′-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,723 bases in length with a single open reading frame extending from nucleotides 97 to 10,269 encoding 3391 amino acids. The 3′-noncoding extremity presents a stem- and loop-structure and contains a repeated oligonucleotide sequence. Comparisons of the nucleotide sequences of the genomes of dengue 2 viruses of different topotypes reveal 90-95% similarity, with 64-66% similarity evident between dengue viruses of different serotypes. The amino acid sequence of the polyprotein of dengue 2 Jamaica virus shows 97, 68, 50, and 44% similarity with those of other dengue 2, dengue 1, or dengue 4, West Nile, and yellow fever viruses, respectively. Despite amino acid sequence divergence, the hydrophobic profile of the flavivirus proteins is highly conserved. Proteins NS1, NS3, and NS5 are the most conserved. Conserved amino acid stretches present in all flavivirus proteins may be involved in common essential biological functions.
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U2 - 10.1016/0042-6822(88)90677-0
DO - 10.1016/0042-6822(88)90677-0
M3 - Article
C2 - 3388770
AN - SCOPUS:0024041312
VL - 165
SP - 234
EP - 244
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -