Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype

Vincent Deubel, Richard M. Kinney, Dennis W. Trent

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42%, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43% homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47% homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.

Original languageEnglish (US)
Pages (from-to)365-377
Number of pages13
JournalVirology
Volume155
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Jamaica
Dengue Virus
Amino Acid Sequence
Genotype
West Nile virus
Dengue
Yellow Fever
Proteins
Flavivirus
Cysteine
Yellow fever virus
Viral Structural Proteins
Polyproteins
Amino Acid Sequence Homology
Capsid
Capsid Proteins
Protein C
Open Reading Frames
Membrane Proteins
Nucleotides

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype. / Deubel, Vincent; Kinney, Richard M.; Trent, Dennis W.

In: Virology, Vol. 155, No. 2, 1986, p. 365-377.

Research output: Contribution to journalArticle

Deubel, Vincent ; Kinney, Richard M. ; Trent, Dennis W. / Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype. In: Virology. 1986 ; Vol. 155, No. 2. pp. 365-377.
@article{4bce2fc0c4d34864990eb5af4ff56123,
title = "Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype",
abstract = "The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42{\%}, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43{\%} homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47{\%} homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.",
author = "Vincent Deubel and Kinney, {Richard M.} and Trent, {Dennis W.}",
year = "1986",
doi = "10.1016/0042-6822(86)90200-X",
language = "English (US)",
volume = "155",
pages = "365--377",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype

AU - Deubel, Vincent

AU - Kinney, Richard M.

AU - Trent, Dennis W.

PY - 1986

Y1 - 1986

N2 - The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42%, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43% homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47% homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.

AB - The nucleotide sequence of the 5′-terminal 2469 bases of dengue 2 (Jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and West Nile viruses, which belong to different flavivirus serogroups. The cDNA clone which was sequenced contains a 5′-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5′-C-prM(M)-E-3′ and the beginning of the NS1 nonstructural protein. The amino acid sequence homology between the structural polyprotein precursor of dengue 2 virus and those of yellow fever and West Nile viruses is 36.5 and 42%, respectively. The dengue virus structural proteins are similar in size and composition to those of the other flaviviruses. The basic capsid protein and the membrane and envelope proteins have hydrophobic regions at their C termini. The dengue 2 capsid C, membrane M, and envelope E proteins share 13, 36, and 43% homology, respectively, with the cognate proteins of yellow fever virus, and 33, 32, and 47% homology with the cognate proteins of West Nile virus. All 6 cysteine residues in the dengue 2 premembrane protein and all 12 cysteine residues in the dengue 2 envelope protein are conserved in the cognate proteins of yellow fever and West Nile viruses.

UR - http://www.scopus.com/inward/record.url?scp=0022891635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022891635&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(86)90200-X

DO - 10.1016/0042-6822(86)90200-X

M3 - Article

VL - 155

SP - 365

EP - 377

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -