Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae.

P. Reynolds, L. Prakash, D. Dumais, G. Perozzi, S. Prakash

Research output: Contribution to journalArticle

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Abstract

The RAD10 gene is one of several genes in Saccharomyces cerevisiae required for incision of u.v.-irradiated or cross-linked DNA. We have determined the nucleotide sequence of the RAD10 gene and its flanking regions. The RAD10 nucleotide sequence presented here differs significantly from that recently reported. The RAD10 protein predicted from the nucleotide sequence contains 210 amino acids with a calculated mol. wt. of 24 310. The middle portion of the RAD10 protein, which is highly basic and also contains eight of the total of 10 tyrosine residues present in the protein, may be involved in DNA binding by ionic interactions and tyrosine intercalation between the bases of DNA. A genomic deletion of the entire RAD10 gene does not affect viability; however, the rad10 deletion mutant is highly u.v. sensitive.

Original languageEnglish (US)
Pages (from-to)3549-3552
Number of pages4
JournalThe EMBO journal
Volume4
Issue number13 A
StatePublished - Dec 16 1985

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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