Numbers of synapses in laminae I-IV of the rat dorsal horn

D. L. McNeill, K. Chung, C. E. Hulsebosch, R. P. Bolender, R. E. Coggeshall

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present study determines numerical densities (N(Vsyn)) and total numbers of synaptic discs in laminae I-IV of the rat S2 dorsal horn. Previous methods for N(Vsyn) have the advantage of being relatively simple, but these assume that the discs are round, flat, and of uniform size. In our material, serial reconstructions indicate that these assumptions are not met. Accordingly we use a stereological method that is not as dependent on these assumptions. This method is to divide the surface density of the discs by the mean surface area of a disc (N(Vsyn) - S(Vsyn)/S̄(syn)). We refer to this as a reconstruction method because synaptic discs are reconstructed from serial sections. We also calculate numerical densities by several previously used standard methods, and the findings are similar but not identical. We find that numerical density and total synaptic numbers are smallest in lamina I, and densities and total numbers are not significantly different when lamina II is compared to laminae III and IV. Thus the intense labeling of terminals with certain compounds that characterize lamina I and II does not imply an increase in total synaptic numbers or in synaptic density. In addition there is a general increase in synaptic densities and numbers as one proceeds from lamina I to lamina IV. Another point is that the numerical density of synapses in the dorsal horn is approximately that of the cerebral cortex. These data will serve as a basis from which to judge the effects of denervations and other manipulations that purportedly change synaptic numbers.

Original languageEnglish (US)
Pages (from-to)453-460
Number of pages8
JournalJournal of Comparative Neurology
Volume278
Issue number3
StatePublished - 1988

Fingerprint

Synapses
Substantia Gelatinosa
Spinal Cord Dorsal Horn
Denervation
Cerebral Cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

McNeill, D. L., Chung, K., Hulsebosch, C. E., Bolender, R. P., & Coggeshall, R. E. (1988). Numbers of synapses in laminae I-IV of the rat dorsal horn. Journal of Comparative Neurology, 278(3), 453-460.

Numbers of synapses in laminae I-IV of the rat dorsal horn. / McNeill, D. L.; Chung, K.; Hulsebosch, C. E.; Bolender, R. P.; Coggeshall, R. E.

In: Journal of Comparative Neurology, Vol. 278, No. 3, 1988, p. 453-460.

Research output: Contribution to journalArticle

McNeill, DL, Chung, K, Hulsebosch, CE, Bolender, RP & Coggeshall, RE 1988, 'Numbers of synapses in laminae I-IV of the rat dorsal horn', Journal of Comparative Neurology, vol. 278, no. 3, pp. 453-460.
McNeill DL, Chung K, Hulsebosch CE, Bolender RP, Coggeshall RE. Numbers of synapses in laminae I-IV of the rat dorsal horn. Journal of Comparative Neurology. 1988;278(3):453-460.
McNeill, D. L. ; Chung, K. ; Hulsebosch, C. E. ; Bolender, R. P. ; Coggeshall, R. E. / Numbers of synapses in laminae I-IV of the rat dorsal horn. In: Journal of Comparative Neurology. 1988 ; Vol. 278, No. 3. pp. 453-460.
@article{eda98a34ed344b5da7c1b783b05c1ccb,
title = "Numbers of synapses in laminae I-IV of the rat dorsal horn",
abstract = "The present study determines numerical densities (N(Vsyn)) and total numbers of synaptic discs in laminae I-IV of the rat S2 dorsal horn. Previous methods for N(Vsyn) have the advantage of being relatively simple, but these assume that the discs are round, flat, and of uniform size. In our material, serial reconstructions indicate that these assumptions are not met. Accordingly we use a stereological method that is not as dependent on these assumptions. This method is to divide the surface density of the discs by the mean surface area of a disc (N(Vsyn) - S(Vsyn)/S̄(syn)). We refer to this as a reconstruction method because synaptic discs are reconstructed from serial sections. We also calculate numerical densities by several previously used standard methods, and the findings are similar but not identical. We find that numerical density and total synaptic numbers are smallest in lamina I, and densities and total numbers are not significantly different when lamina II is compared to laminae III and IV. Thus the intense labeling of terminals with certain compounds that characterize lamina I and II does not imply an increase in total synaptic numbers or in synaptic density. In addition there is a general increase in synaptic densities and numbers as one proceeds from lamina I to lamina IV. Another point is that the numerical density of synapses in the dorsal horn is approximately that of the cerebral cortex. These data will serve as a basis from which to judge the effects of denervations and other manipulations that purportedly change synaptic numbers.",
author = "McNeill, {D. L.} and K. Chung and Hulsebosch, {C. E.} and Bolender, {R. P.} and Coggeshall, {R. E.}",
year = "1988",
language = "English (US)",
volume = "278",
pages = "453--460",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Numbers of synapses in laminae I-IV of the rat dorsal horn

AU - McNeill, D. L.

AU - Chung, K.

AU - Hulsebosch, C. E.

AU - Bolender, R. P.

AU - Coggeshall, R. E.

PY - 1988

Y1 - 1988

N2 - The present study determines numerical densities (N(Vsyn)) and total numbers of synaptic discs in laminae I-IV of the rat S2 dorsal horn. Previous methods for N(Vsyn) have the advantage of being relatively simple, but these assume that the discs are round, flat, and of uniform size. In our material, serial reconstructions indicate that these assumptions are not met. Accordingly we use a stereological method that is not as dependent on these assumptions. This method is to divide the surface density of the discs by the mean surface area of a disc (N(Vsyn) - S(Vsyn)/S̄(syn)). We refer to this as a reconstruction method because synaptic discs are reconstructed from serial sections. We also calculate numerical densities by several previously used standard methods, and the findings are similar but not identical. We find that numerical density and total synaptic numbers are smallest in lamina I, and densities and total numbers are not significantly different when lamina II is compared to laminae III and IV. Thus the intense labeling of terminals with certain compounds that characterize lamina I and II does not imply an increase in total synaptic numbers or in synaptic density. In addition there is a general increase in synaptic densities and numbers as one proceeds from lamina I to lamina IV. Another point is that the numerical density of synapses in the dorsal horn is approximately that of the cerebral cortex. These data will serve as a basis from which to judge the effects of denervations and other manipulations that purportedly change synaptic numbers.

AB - The present study determines numerical densities (N(Vsyn)) and total numbers of synaptic discs in laminae I-IV of the rat S2 dorsal horn. Previous methods for N(Vsyn) have the advantage of being relatively simple, but these assume that the discs are round, flat, and of uniform size. In our material, serial reconstructions indicate that these assumptions are not met. Accordingly we use a stereological method that is not as dependent on these assumptions. This method is to divide the surface density of the discs by the mean surface area of a disc (N(Vsyn) - S(Vsyn)/S̄(syn)). We refer to this as a reconstruction method because synaptic discs are reconstructed from serial sections. We also calculate numerical densities by several previously used standard methods, and the findings are similar but not identical. We find that numerical density and total synaptic numbers are smallest in lamina I, and densities and total numbers are not significantly different when lamina II is compared to laminae III and IV. Thus the intense labeling of terminals with certain compounds that characterize lamina I and II does not imply an increase in total synaptic numbers or in synaptic density. In addition there is a general increase in synaptic densities and numbers as one proceeds from lamina I to lamina IV. Another point is that the numerical density of synapses in the dorsal horn is approximately that of the cerebral cortex. These data will serve as a basis from which to judge the effects of denervations and other manipulations that purportedly change synaptic numbers.

UR - http://www.scopus.com/inward/record.url?scp=0024263135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024263135&partnerID=8YFLogxK

M3 - Article

VL - 278

SP - 453

EP - 460

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -