Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales

Kathie A. Mihindukulasuriya, Nang L. Nguyen, Guang Wu, Henry V. Huang, Amelia P A Travassos Da Rosa, Vsevolod Popov, Robert B. Tesh, David Wang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Here, we report the sequencing and classification of Nyamanini virus (NYMV) and Midway virus (MIDWV), two antigenically related viruses that were first isolated in 1957 and 1966, respectively. Although these viruses have been cultured multiple times from cattle egrets, seabirds, and their ticks, efforts to classify them taxonomically using conventional serological and electron microscopic approaches have failed completely. We used a random shotgun sequencing strategy to define the genomes of NYMV and MIDWV. Contigs of 11,631 and 11,752 nucleotides, representing the complete genome of NYMV and the near-complete genome of MIDWV, respectively, were assembled. Each virus genome was predicted to carry six open reading frames (ORFs). BLAST analysis indicated that only two of the ORF proteins of each virus, the putative nucleocapsid and polymerase, had detectable sequence similarity to known viral proteins. Phylogenetic analysis of these ORF proteins demonstrated that the closest relatives of NYNV and MIDWV are negative-stranded-RNA viruses in the order Mononegavirales. On the basis of their very limited sequence similarity to known viruses, we propose that NYMV and MIDWV define a novel genus, Nyavirus, in this order.

Original languageEnglish (US)
Pages (from-to)5109-5116
Number of pages8
JournalJournal of Virology
Volume83
Issue number10
DOIs
StatePublished - May 2009

Fingerprint

Mononegavirales
RNA Viruses
Nyamanini virus
Viruses
viruses
open reading frames
genome
Genome
Open Reading Frames
RNA viruses
Bubulcus ibis
nucleocapsid
viral proteins
seabirds
Nucleocapsid

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Mihindukulasuriya, K. A., Nguyen, N. L., Wu, G., Huang, H. V., Travassos Da Rosa, A. P. A., Popov, V., ... Wang, D. (2009). Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales. Journal of Virology, 83(10), 5109-5116. https://doi.org/10.1128/JVI.02667-08

Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales. / Mihindukulasuriya, Kathie A.; Nguyen, Nang L.; Wu, Guang; Huang, Henry V.; Travassos Da Rosa, Amelia P A; Popov, Vsevolod; Tesh, Robert B.; Wang, David.

In: Journal of Virology, Vol. 83, No. 10, 05.2009, p. 5109-5116.

Research output: Contribution to journalArticle

Mihindukulasuriya, KA, Nguyen, NL, Wu, G, Huang, HV, Travassos Da Rosa, APA, Popov, V, Tesh, RB & Wang, D 2009, 'Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales', Journal of Virology, vol. 83, no. 10, pp. 5109-5116. https://doi.org/10.1128/JVI.02667-08
Mihindukulasuriya KA, Nguyen NL, Wu G, Huang HV, Travassos Da Rosa APA, Popov V et al. Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales. Journal of Virology. 2009 May;83(10):5109-5116. https://doi.org/10.1128/JVI.02667-08
Mihindukulasuriya, Kathie A. ; Nguyen, Nang L. ; Wu, Guang ; Huang, Henry V. ; Travassos Da Rosa, Amelia P A ; Popov, Vsevolod ; Tesh, Robert B. ; Wang, David. / Nyamanini and midway viruses define a novel taxon of RNA viruses in the order Mononegavirales. In: Journal of Virology. 2009 ; Vol. 83, No. 10. pp. 5109-5116.
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