Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

Yan Liu, Zhikai Zhang, Yongquan Jiang, Lihong Zhang, Vsevolod Popov, Jianzhi Zhang, David Walker, Xue jie Yu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalMicrobes and Infection
Volume13
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Ehrlichia
Bacteria
Mitochondria
Phagocytes
Ehrlichia chaffeensis
Morula
Mitochondrial Genes
Bromodeoxyuridine
Vacuoles
Confocal Microscopy
Cytoplasm

Keywords

  • Ehrlichia
  • Ehrlichiosis
  • Mitochondria

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity. / Liu, Yan; Zhang, Zhikai; Jiang, Yongquan; Zhang, Lihong; Popov, Vsevolod; Zhang, Jianzhi; Walker, David; Yu, Xue jie.

In: Microbes and Infection, Vol. 13, No. 3, 03.2011, p. 232-238.

Research output: Contribution to journalArticle

Liu, Yan ; Zhang, Zhikai ; Jiang, Yongquan ; Zhang, Lihong ; Popov, Vsevolod ; Zhang, Jianzhi ; Walker, David ; Yu, Xue jie. / Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity. In: Microbes and Infection. 2011 ; Vol. 13, No. 3. pp. 232-238.
@article{e6677ca6d3114ce7a1ae64d6456708e1,
title = "Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity",
abstract = "Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.",
keywords = "Ehrlichia, Ehrlichiosis, Mitochondria",
author = "Yan Liu and Zhikai Zhang and Yongquan Jiang and Lihong Zhang and Vsevolod Popov and Jianzhi Zhang and David Walker and Yu, {Xue jie}",
year = "2011",
month = "3",
doi = "10.1016/j.micinf.2010.10.021",
language = "English (US)",
volume = "13",
pages = "232--238",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "3",

}

TY - JOUR

T1 - Obligate intracellular bacterium Ehrlichia inhibiting mitochondrial activity

AU - Liu, Yan

AU - Zhang, Zhikai

AU - Jiang, Yongquan

AU - Zhang, Lihong

AU - Popov, Vsevolod

AU - Zhang, Jianzhi

AU - Walker, David

AU - Yu, Xue jie

PY - 2011/3

Y1 - 2011/3

N2 - Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

AB - Ehrlichia are obligately intracellular bacteria that reside in a vacuole in the cytoplasm of phagocytes. We determined by confocal microscopy the interaction between Ehrlichia and mitochondria in DH82 cells to investigate the mechanism of Ehrlichia survival inside the phagocyte. The most remarkable finding of our study was that Ehrlichia morulae interacted with mitochondria and inhibited mitochondrial metabolism. We showed that in Ehrlichia chaffeensis-infected DH82 cells, mitochondria did not incorporate BrdU and transcriptional level of the mitochondrial gene NADPH2 was significantly reduced, indicating the inhibition of mitochondrial metabolism. This study demonstrates that Ehrlichia are able to inhibit mitochondrial activities, and it opens up a new avenue for the study of Ehrlichia pathogenesis.

KW - Ehrlichia

KW - Ehrlichiosis

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=78951491174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78951491174&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2010.10.021

DO - 10.1016/j.micinf.2010.10.021

M3 - Article

VL - 13

SP - 232

EP - 238

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 3

ER -