Observations on prostaglandins in normal and leukemic human lymphocytes

Ugo Carpentieri, Ben H. Brouhard, Lavenia LaGrone, Lillian H. Lockhart

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Prostaglandins E (PGE) and F (PGF) were measured in lymphocytes of normal subjects, children with acute lymphocytic leukemia (ALL), and adults with chronic lymphocytic leukemia (CLL). In ALL lymphocytes PGE increased from a normal value of 25 pgrams to 270 pgrams/106 cells, and PGF increased from a normal value of 31 pgrams to 482 pgrams/106 cells. In CLL lymphocytes, levels of PGE and PGF were normal or low. When normal lymphocytes were stimulated with phytohemagglutinin (PHA), the level of PGE and PGF fluctuated, followed by corresponding changes in the level of cyclic nucleotides. In cultured ALL lymphocytes, the level of PGE remained high, while cyclic 3′:5′-adenosine monophosphate (c-AMP) level was constantly low, and the initial high level of PGF fluctuated in relation to similar oscillations of cyclic 3′:5′-guanosine monophosphate (c-GMP). These values were lower, although not significantly, when ALL lymphocytes were stimulated with PHA. When CLL lymphocytes were stimulated with PHA, the level of PGE remained low (20 pgrams), as did that of c-AMP. The level of PGF, after a brief initial increase (130 pgrams), returned to and remained at a lower level (60 pgrams) while the level of c-GMP was persistently high. These results suggest: (1) prostaglandins may indirectly influence the cell cycle, possibly through modulation of cyclase activity and levels of cyclic nucleotides; and (2) some derangement of this regulatory mechanism may be present in leukemic lymphocytes.

Original languageEnglish (US)
Pages (from-to)1117-1129
Number of pages13
JournalProstaglandins
Volume20
Issue number6
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Fingerprint Dive into the research topics of 'Observations on prostaglandins in normal and leukemic human lymphocytes'. Together they form a unique fingerprint.

  • Cite this

    Carpentieri, U., Brouhard, B. H., LaGrone, L., & Lockhart, L. H. (1980). Observations on prostaglandins in normal and leukemic human lymphocytes. Prostaglandins, 20(6), 1117-1129. https://doi.org/10.1016/0090-6980(80)90064-7